Pozzan T, Lew D P, Wollheim C B, Tsien R Y
Science. 1983 Sep 30;221(4618):1413-5. doi: 10.1126/science.6310757.
The concentration of cytosolic ionized calcium, [Ca2+]i, was measured in intact neutrophils by use of a fluorescent indicator trapped in the icytoplasm. A given rise of [Ca2+]i elicited by the chemotactic peptide formylmethionylleucylphenylalanine (FMLP) was associated with a much greater degree of superoxide generation and myeloperoxidase secretion than was the same or larger [Ca2+]i produced by a specific calcium ionophore, ionomycin, which bypasses cell surface receptors. Thus, FMLP appears to generate some important excitatory signal in addition to a rise in [Ca2+]i and exocytosis and superoxide generation in neutrophils may not be simply dependent on [Ca2+]i as is widely supposed.
通过使用被困在细胞质中的荧光指示剂，在完整的中性粒细胞中测量细胞质游离钙离子浓度[Ca2+]i。趋化肽甲酰甲硫氨酰亮氨酰苯丙氨酸（FMLP）引起的[Ca2+]i给定升高，与超氧化物生成和髓过氧化物酶分泌程度相比，比由特异性钙离子载体离子霉素产生的相同或更高的[Ca2+]i所引起的程度要大得多，离子霉素绕过细胞表面受体。因此，FMLP似乎除了引起[Ca2+]i升高外，还产生一些重要的兴奋性信号，并且中性粒细胞中的胞吐作用和超氧化物生成可能不像广泛认为的那样简单地依赖于[Ca2+]i。
