Goldstone A D, Koenig H, Lu C Y, Trout J J
Biochem Biophys Res Commun. 1983 Aug 12;114(3):913-21. doi: 10.1016/0006-291x(83)90647-2.
The beta-adrenergic agonist 1-isoproterenol evokes an acute (less than 5 min) stimulation of endocytosis, hexose transport and amino acid transport, measured by the temperature-sensitive uptake of HRP, 3H-DG and 14C-AIB, in mouse kidney cortex slices. This stimulation is concentration dependent and is maximal at 10(-8)-10(-7) M isoproterenol. Peroxidase cytochemistry showed that the hormonal increase in HRP uptake is confined to proximal tubules. The rapid membrane response is abolished in a calcium-free medium and by the beta-adrenergic antagonist propranolol, indicating Ca2+- and beta-adrenoreceptor-dependence. Isoproterenol (1 microM) rapidly (less than 30 sec) stimulates the influx and efflux of 45Ca in cortex slices. Isoproterenol also decreased mitochondrial 45Ca and increased soluble 45Ca. These results indicate that beta-adrenergic stimulation of membrane transport functions involves an increased influx of extracellular calcium and a mobilization of intracellular (mitochondrial) calcium. An increase in cytosolic Ca2+ concentration appears to be the regulatory signal for these membrane transport processes.
β-肾上腺素能激动剂1-异丙肾上腺素能引发急性（少于5分钟）的内吞作用、己糖转运和氨基酸转运刺激，这通过小鼠肾皮质切片中辣根过氧化物酶（HRP）、3H-脱氧葡萄糖（3H-DG）和14C-α-氨基异丁酸（14C-AIB）的温度敏感性摄取来测量。这种刺激呈浓度依赖性，在10^(-8)-10^(-7) M异丙肾上腺素时达到最大值。过氧化物酶细胞化学显示，HRP摄取的激素增加仅限于近端小管。在无钙培养基中以及通过β-肾上腺素能拮抗剂普萘洛尔可消除这种快速的膜反应，表明其依赖于Ca2+和β-肾上腺素受体。异丙肾上腺素（1 μM）能迅速（少于30秒）刺激皮质切片中45Ca的流入和流出。异丙肾上腺素还能降低线粒体45Ca并增加可溶性45Ca。这些结果表明，β-肾上腺素能对膜转运功能的刺激涉及细胞外钙流入增加和细胞内（线粒体）钙的动员。细胞质Ca2+浓度的增加似乎是这些膜转运过程的调节信号。
