Likhtenshteĭn G I, Bogatyrenko V R, Kulikov A V
Biofizika. 1983 Jul-Aug;28(4):585-9.
This paper presents experimental results of inner dynamics study of human serum albumin by a series of physical methods: a) the method of radical pairs recombination based on measuring of recombination rate of radical pairs included to surface layers of a protein globule; b) the method of spin labels with saturation transfer technique. The complex of methods used in this work permits to measure the mobility of physical labels and environment matrix in a wide correlation frequency range 10(-4)-5.10(10) s-1 in 30-230 K temperature range. The analysis of the data obtained allows to make conclusions on the molecular mobility in surface layers of a protein globule under different conditions.
a）基于测量包含在蛋白质球表面层中的自由基对复合率的自由基对复合方法；b）采用饱和转移技术的自旋标记法。本研究中使用的方法组合能够在30 - 230 K的温度范围内，在10⁻⁴ - 5×10¹⁰ s⁻¹的宽相关频率范围内测量物理标记和环境基质的流动性。对所获得数据的分析有助于得出不同条件下蛋白质球表面层分子流动性的结论。
