McCarty J M, Grant C K
Cell Immunol. 1983 Oct 1;81(1):157-68. doi: 10.1016/0008-8749(83)90221-6.
Humoral and cellular cytotoxic immune mechanisms of cats were compared against feline leukemia virus (FeLV)- and feline sarcoma virus (FeSV)-transformed cells. The groups of animals studied were nonexposed control cats; FeLV-infected immune or viremic tumor-bearing cats; FeSV-inoculated tumor progressor or regressor cats, and cats immunized with FeSV-transformed autochthonous fibroblasts (ATF). Sera containing complement-dependent antibodies (CDA), which lysed FeLV-producer lymphoma lines, had no cytotoxic effects when tested against FeLV-producer FeSV-transformed fibroblasts. Sera with lytic CDA activity were also tested for antibody-dependent cellular cytotoxic (ADCC) effects with peripheral blood lymphocytes (PBL) from nonimmune cats. No ADCC activity was detected against either lymphoid or fibroblast target lines. To demonstrate that cat PBL contained ADCC effector cells, antibody-coated murine target cells were employed and positive results obtained. Natural killer (NK) assays were performed using PBL from normal and tumor-bearing cats. Cytotoxic effects were only detectable to FeLV-producer lymphomas, and comparable levels of NK activity were found in normal and lymphoid tumor-bearing animals. In cats immunized with ATF, a population of effector cells was found in peripheral blood which had functional characteristics of cytotoxic T lymphocytes (CTL). The killing of ATF by CTL-like cells was not inhibited by FeLV/FeSV immune sera or by sera from autochthonous immune cats. The comparative importance of humoral and cellular cytotoxic mechanisms against FeLV- and FeSV-induced tumors is discussed.
对猫的体液和细胞细胞毒性免疫机制针对猫白血病病毒（FeLV）和猫肉瘤病毒（FeSV）转化细胞进行了比较。所研究的动物组包括未接触过病毒的对照猫；感染FeLV的免疫或病毒血症的荷瘤猫；接种FeSV的肿瘤进展或消退猫，以及用FeSV转化的自体成纤维细胞（ATF）免疫的猫。含有能裂解FeLV产生性淋巴瘤细胞系的补体依赖性抗体（CDA）的血清，在针对FeLV产生性FeSV转化的成纤维细胞进行检测时没有细胞毒性作用。还使用来自非免疫猫的外周血淋巴细胞（PBL）对具有溶细胞CDA活性的血清进行了抗体依赖性细胞毒性（ADCC）作用检测。未检测到针对淋巴细胞或成纤维细胞靶细胞系的ADCC活性。为了证明猫PBL含有ADCC效应细胞，使用了抗体包被的鼠靶细胞并获得了阳性结果。使用来自正常和荷瘤猫的PBL进行了自然杀伤（NK）检测。仅对FeLV产生性淋巴瘤可检测到细胞毒性作用，并且在正常和荷瘤淋巴组织的动物中发现了相当水平的NK活性。在用ATF免疫的猫中，在外周血中发现了一群具有细胞毒性T淋巴细胞（CTL）功能特征的效应细胞。CTL样细胞对ATF的杀伤作用不受FeLV/FeSV免疫血清或自体免疫猫血清的抑制。讨论了针对FeLV和FeSV诱导肿瘤的体液和细胞细胞毒性机制的相对重要性。
