Hennig E E, Demkowicz-Dobrzański K K, Sawicki J T, Mojska H, Kujawa M
Carcinogenesis. 1983 Oct;4(10):1243-6. doi: 10.1093/carcin/4.10.1243.
The effect of butylated hydroxyanisole (BHA) administration on the hepatic monooxygenase system of nuclear and microsomal fraction was investigated in male mice. Addition of BHA to the diet significantly lowered the content of cytochrome P-450 in liver nuclei and increased the specific activity of NADPH-cytochrome c reductase and the content of cytochrome b5 in liver microsomes. Incubation of benzo[a]pyrene (BP) with liver nuclei from BHA-fed mice resulted in inhibition of binding of BP metabolites to nuclear macromolecules by 50% compared with control. However, there was no effect of BHA on the binding of BP metabolites to macromolecules when BP was incubated with added DNA and liver microsomes from BHA-fed mice. It has been postulated that modification of nuclear monooxygenases by BHA may play a role in the inhibitory effect of BHA on BP carcinogenesis.
研究了丁基羟基茴香醚（BHA）给药对雄性小鼠肝脏细胞核和微粒体部分单加氧酶系统的影响。在饮食中添加BHA可显著降低肝细胞核中细胞色素P-450的含量，并增加肝微粒体中NADPH-细胞色素c还原酶的比活性和细胞色素b5的含量。与对照组相比，用BHA喂养小鼠的肝细胞核与苯并[a]芘（BP）孵育后，BP代谢产物与核大分子的结合受到50%的抑制。然而，当BP与添加的DNA和用BHA喂养小鼠的肝微粒体孵育时，BHA对BP代谢产物与大分子的结合没有影响。据推测，BHA对细胞核单加氧酶的修饰可能在BHA对BP致癌作用的抑制效应中发挥作用。
