Filipski J, Yin J, Kohn K W
Enzyme. 1983;30(2):89-94. doi: 10.1159/000469555.
Topoisomerase was extracted from L-1210 nuclei by 0.35 M sodium chloride Extract fractionation by molecular weight revealed two activities. The activities were quantitatively determined using a technique based on the electrophoretic separation of 3H-labelled DNA product from substrate. This technique enables the determination of topoisomerase activity in extracts where nuclease activity is also present.
通过0.35M氯化钠从L - 1210细胞核中提取拓扑异构酶。按分子量对提取物进行分级分离显示出两种活性。使用基于从底物中电泳分离3H标记的DNA产物的技术对这些活性进行定量测定。该技术能够测定同时存在核酸酶活性的提取物中的拓扑异构酶活性。
