Paietta E, Schwarzmeier J D
Eur J Clin Invest. 1983 Aug;13(4):339-46. doi: 10.1111/j.1365-2362.1983.tb00110.x.
An identical class of high-affinity binding sites for the 125I-labelled beta-adrenergic antagonist hydroxybenzylpindolol, was identified on intact human normal and leukaemic peripheral blood leukocytes. On normal unfractionated lymphocytes, polymorphonuclear leukocytes, and monocytes, receptor density did not differ significantly (1200-1400 receptors per cell; P greater than 0.3), but it was higher on B- than on T-lymphocytes (P less than 0.05). In leukaemia, monocytic blast cells expressed highest receptor numbers, whereas very low receptor density was seen on the pathologic B-cells from chronic lymphocytic leukaemia. Among normal leukocytes, adenylate cyclase activation by hormones (isoproterenol, prostaglandin E1, histamine) and sodium fluoride was strongest in plasma membranes from monocytes, but very weak in polymorphonuclear leukocytes either due to uncoupling of hormone receptors from adenylate cyclase or to low catalytic activity. In T-cells, enzyme activity was significantly lower than in B-cells. Loss of adenylate cyclase sensitivity to hormones and fluoride occurred in leukaemic cells from chronic and acute lymphocytic leukaemia.
在完整的人类正常外周血白细胞和白血病外周血白细胞上，鉴定出了一类相同的、对125I标记的β-肾上腺素能拮抗剂羟基苄基吲哚洛尔具有高亲和力的结合位点。在正常未分离的淋巴细胞、多形核白细胞和单核细胞上，受体密度没有显著差异（每个细胞1200 - 1400个受体；P大于0.3），但B淋巴细胞上的受体密度高于T淋巴细胞（P小于0.05）。在白血病中，单核细胞母细胞表达的受体数量最高；而慢性淋巴细胞白血病的病理性B细胞上的受体密度非常低。在正常白细胞中，激素（异丙肾上腺素、前列腺素E1、组胺）和氟化钠激活腺苷酸环化酶最强的是单核细胞膜，但多形核白细胞中则非常弱，这要么是由于激素受体与腺苷酸环化酶解偶联所致；要么就是由于催化活性低。在T细胞中，酶活性显著低于B细胞。慢性和急性淋巴细胞白血病的白血病细胞中，腺苷酸环化酶对激素和氟化物的敏感性丧失了。
