Fabbro D, Jochum A, Balerna M, Campana A, Eppenberger U
Int J Androl. 1983 Aug;6(4):337-48. doi: 10.1111/j.1365-2605.1983.tb00548.x.
Photoaffinity labelling by 8-N3-/32P/cAMP of human sperm homogenates with normal pathology and normal progressive motility of the gametes (N) revealed the presence of 2 specific cAMP-binding activities with MW 47 000 (R-I) and 52 000 (R-II). Similar amounts of R-I and R-II were found in sperm homogenates obtained from semen samples with either reduced progressive motility (A50: 30-50% progressive motility and A30: less than 30% progressive motility) or abnormal morphology of the gametes (T: 5-30% normal heads). In contrast, the seminal plasma of the respective semen samples of N, A50, A30 and T incorporated the 8-azido cAMP photolabel in similar quantities into 2 additional cAMP-binding proteins with MW 42 000 and 37 000. Quantitative analysis of the photoaffinity labelling of the seminal plasma obtained from normal (N) or subnormal semen (A50, A30, T) showed that cAMP was predominantly bound to the 37 000 protein. Furthermore, the cAMP-dependent protein kinase and cAMP-binding activities of sperm homogenates were quantitatively comparable within the 4 groups. These results provide the first evidence that neither quantitative nor qualitative differences in the molecular and/or enzymatic properties of cAMP-dependent protein kinases can be detected between normal human sperm (N) and those with either reduced progressive motility (A50, A30) or abnormal morphology (T).
用8-N3-/32P/cAMP对具有正常病理学和正常配子前向运动能力（N）的人精子匀浆进行光亲和标记，结果显示存在两种特异性的cAMP结合活性，分子量分别为47 000（R-I）和52 000（R-II）。在从具有降低的前向运动能力（A50：前向运动能力为30%-50%和A30：前向运动能力小于30%）或配子形态异常（T：正常头部比例为5%-30%）的精液样本中获得的精子匀浆中，发现了相似数量的R-I和R-II。相比之下，N、A50、A30和T各自精液样本的精浆将8-叠氮基cAMP光标记以相似的量掺入另外两种分子量分别为42 000和37 000的cAMP结合蛋白中。对从正常（N）或亚正常精液（A50、A30、T）获得的精浆的光亲和标记进行定量分析表明，cAMP主要与分子量为37 000的蛋白结合。此外，精子匀浆的cAMP依赖性蛋白激酶和cAMP结合活性在这4组中在数量上具有可比性。这些结果提供了首个证据，即在正常人类精子（N）与那些具有降低的前向运动能力（A50、A30）或异常形态（T）的精子之间，未检测到cAMP依赖性蛋白激酶的分子和/或酶学性质在数量或质量上的差异。
