Chua C C, Geiman D, Ladda R L
J Cell Physiol. 1983 Oct;117(1):116-22. doi: 10.1002/jcp.1041170116.
Kirsten murine sarcoma virus (KiMSV)-transformed rat kidney cells (KNRK) release small polypeptides (Mr 12,500-15,300) into the culture medium that are capable of stimulating normal rat kidney cells (NRK) to form colonies in soft agar. The transforming growth factors (TGFs) did not compete with epidermal growth factor (EGF) for its receptor and did not induce specific phosphorylation of EGF receptor on NRK cell membranes. These properties differ from the TGFs isolated by other investigators. Our data further establish the heterogeneity of the materials produced by transformed cells that induce transformation-specific changes in normal cells.
Kirsten鼠肉瘤病毒（KiMSV）转化的大鼠肾细胞（KNRK）向培养基中释放小分子量多肽（Mr 12,500 - 15,300），这些多肽能够刺激正常大鼠肾细胞（NRK）在软琼脂中形成集落。转化生长因子（TGFs）不与表皮生长因子（EGF）竞争其受体，也不诱导NRK细胞膜上EGF受体的特异性磷酸化。这些特性与其他研究者分离出的TGFs不同。我们的数据进一步证实了转化细胞产生的、能在正常细胞中诱导特异性转化变化的物质具有异质性。
