Yamamura K, Nakao M, Yamada J, Yotsuyanagi T
J Pharm Sci. 1983 Aug;72(8):958-60. doi: 10.1002/jps.2600720833.
A high-performance liquid chromatographic method is described for the simultaneous determinations of cefsulodin and cefotiam in serum and bone marrow blood samples. After extraction with acetonitrile, the cephalosporins were applied to a reverse-phase column with an internal standard, cefazolin; the mobile phase was a mixture of 0.005 M tetrabutylammonium phosphate and methanol (35:65, v/v). The method yielded satisfactory resolutions for these agents, and the results were compared with those obtained using the microbiological method. The statistical analysis of the relationship between the methods gave a good correlation for all of these agents and samples. The concentrations of cefsulodin and cefotiam, concurrently administered by the intravenous route to patients subjected to artificial total joint prosthesis, in serum and bone marrow blood collected at 0.5 and 1 hr postinjection were almost equivalent.
描述了一种高效液相色谱法，用于同时测定血清和骨髓血样中的磺苄西林和头孢替安。用乙腈萃取后，将头孢菌素应用于带有内标头孢唑林的反相柱；流动相为0.005M磷酸四丁铵和甲醇的混合物（35:65，v/v）。该方法对这些药物产生了令人满意的分离效果，并将结果与使用微生物学方法获得的结果进行了比较。对两种方法之间关系的统计分析表明，所有这些药物和样品都具有良好的相关性。对接受人工全关节置换术的患者静脉注射磺苄西林和头孢替安后，在注射后0.5小时和1小时采集的血清和骨髓血中，磺苄西林和头孢替安的浓度几乎相等。
