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冷冻可消除突触膜中特定群体的L-谷氨酸受体。

Freezing eliminates a specific population of L-glutamate receptors in synaptic membranes.

作者信息

Fagg G E, Mena E E, Monaghan D T, Cotman C W

出版信息

Neurosci Lett. 1983 Jul 29;38(2):157-62. doi: 10.1016/0304-3940(83)90033-2.

DOI:10.1016/0304-3940(83)90033-2
PMID:6312382
Abstract

The binding of L-[3H]glutamate (L-Glu) to freeze-thawed synaptic membranes (SPMs) exhibited saturation kinetics, with Kd 507 nM and Bmax 6.99 pmol/mg protein. The effects of ions, the susceptibility to Triton X-100 and the pharmacological properties of the binding indicated that those sites detected in freeze-thawed SPMs were only of the Cl-/Ca2+-independent type. The Cl-/Ca2+-dependent (2-amino-4-phosphonobutyrate-sensitive) L-Glu binding sites which are additionally present in fresh SPMs are abolished by freezing.

摘要

L-[3H]谷氨酸（L-Glu）与冻融突触膜（SPMs）的结合呈现饱和动力学，解离常数（Kd）为507 nM，最大结合量（Bmax）为6.99 pmol/mg蛋白。离子的影响、对Triton X-100的敏感性以及结合的药理学特性表明，在冻融SPMs中检测到的那些位点仅为不依赖Cl-/Ca2+的类型。新鲜SPMs中额外存在的依赖Cl-/Ca2+（对2-氨基-4-膦酰丁酸敏感）的L-Glu结合位点在冷冻后消失。

相似文献

1
Freezing eliminates a specific population of L-glutamate receptors in synaptic membranes.冷冻可消除突触膜中特定群体的L-谷氨酸受体。
Neurosci Lett. 1983 Jul 29;38(2):157-62. doi: 10.1016/0304-3940(83)90033-2.
2
Cl-/Ca2+-dependent L-glutamate binding sites do not correspond to 2-amino-4-phosphonobutanoate-sensitive excitatory amino acid receptors.氯离子/钙离子依赖性L-谷氨酸结合位点与对2-氨基-4-膦酰丁酸敏感的兴奋性氨基酸受体并不对应。
Br J Pharmacol. 1985 Nov;86(3):743-51. doi: 10.1111/j.1476-5381.1985.tb08954.x.
3
Binding of L-[3H]glutamate to fresh or frozen synaptic membrane and postsynaptic density fractions isolated from cerebral cortex and cerebellum of fresh or frozen canine brain.
J Neurochem. 1986 Mar;46(3):831-41. doi: 10.1111/j.1471-4159.1986.tb13047.x.
4
Characterization of L-glutamate binding sites in rat spinal cord synaptic membranes: evidence for multiple chloride ion-dependent sites.大鼠脊髓突触膜中L-谷氨酸结合位点的表征：多个氯离子依赖性位点的证据。
J Neurochem. 1986 Oct;47(4):1052-60. doi: 10.1111/j.1471-4159.1986.tb00719.x.
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Sodium ions regulate a specific population of acidic amino acid receptors in synaptic membranes.钠离子调节突触膜中特定群体的酸性氨基酸受体。
Life Sci. 1984 Apr 30;34(18):1739-45. doi: 10.1016/0024-3205(84)90573-3.
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N-methyl-D-aspartate-sensitive [3H]glutamate binding sites in brain synaptic membranes treated with Triton X-100.用Triton X-100处理的脑突触膜中对N-甲基-D-天冬氨酸敏感的[3H]谷氨酸结合位点。
Biochim Biophys Acta. 1989 Jun 15;1012(1):74-80. doi: 10.1016/0167-4889(89)90013-x.
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Characterization of the binding of DL-[3H]-2-amino-4-phosphonobutyrate to L-glutamate-sensitive sites on rat brain synaptic membranes.DL-[3H]-2-氨基-4-膦酰丁酸与大鼠脑突触膜上L-谷氨酸敏感位点结合的特性研究。
Br J Pharmacol. 1983 Oct;80(2):355-64. doi: 10.1111/j.1476-5381.1983.tb10041.x.
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Chloride and calcium ions separate L-glutamate receptor populations in synaptic membranes.
Eur J Pharmacol. 1983 Mar 18;88(1):105-10. doi: 10.1016/0014-2999(83)90397-7.
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A comparative study of L[3H]-glutamate and L[3H]-cysteine sulfinate binding sites in subcellular fractions of rat brain.大鼠脑亚细胞组分中L[3H]-谷氨酸和L[3H]-半胱亚磺酸结合位点的比较研究。
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Disclosure by triton X-100 of NMDA-sensitive [3H] glutamate binding sites in brain synaptic membranes.曲拉通X-100对脑突触膜中NMDA敏感的[³H]谷氨酸结合位点的暴露。
Biochem Biophys Res Commun. 1988 Jun 16;153(2):510-7. doi: 10.1016/s0006-291x(88)81124-0.

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Cl-/Ca2+-dependent L-glutamate binding sites do not correspond to 2-amino-4-phosphonobutanoate-sensitive excitatory amino acid receptors.氯离子/钙离子依赖性L-谷氨酸结合位点与对2-氨基-4-膦酰丁酸敏感的兴奋性氨基酸受体并不对应。
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5
Differentiation of Cl-/Ca2+-dependent and sodium dependent 3H-glutamate binding to cortical membranes from rat brain by high energy radiation inactivation analysis.
J Neural Transm. 1986;65(2):93-101. doi: 10.1007/BF01256485.
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