Nemchinskaia V L, Pokrovskaia T G, Mozhenok T P, Braun A D
Tsitologiia. 1983 Jul;25(7):799-804.
Murine peritoneal macrophages are able to hydrolyse NAD+ and NADP+. The NADPase activity exceeds that of NADase by 22-24%. The pH optima for both the enzymes are, respectively, 6.0 and 7.0. NAD hydrolysis is considerably activated by Mg2+, whereas NADP hydrolysis remains not affected. NAD+ does not change NADPase activity, while NADase activity is inhibited by NADP by 25-30%. A diazonium salt of sulfanilic acid, known to be an inhibitor of cell plasma membranes, does not affect NADP+ hydrolysis and causes a 20-30% retardation of NAD+ hydrolysis. The data obtained suggest that murine peritoneal macrophages contain two hydrolytic enzymes: NADase and NADPase.
小鼠腹腔巨噬细胞能够水解NAD⁺和NADP⁺。NADP酶活性比NAD酶活性高22 - 24%。这两种酶的最适pH分别为6.0和7.0。Mg²⁺能显著激活NAD水解，而NADP水解不受影响。NAD⁺不改变NADP酶活性，而NADP可抑制NAD酶活性25 - 30%。已知对氨基苯磺酸重氮盐是细胞质膜的抑制剂，它不影响NADP⁺水解，却会使NAD⁺水解延迟20 - 30%。所得数据表明，小鼠腹腔巨噬细胞含有两种水解酶：NAD酶和NADP酶。
