Takashima I, Hashimoto N, Arikawa J, Matsumoto K
Arch Virol. 1983;76(4):299-305. doi: 10.1007/BF01311197.
Vector competences of Aedes (Ae.) vexans nipponii (nip.) and Culex (Cx.) tritaeniorhynchus to Getah virus were assessed by using a membrane feeding technique. The Getah virus was present at high titer in both species of mosquitoes after 21 days of extrinsic incubation at 28 degrees C. Infection rates on 21 post-feeding were 100 per cent (4/4) for Ae. vexans nip. at a virus dosage of 10(5.3) PFU/ml and 60 per cent (3/5) for Cx. tritaeniorhynchus at similar virus dosage. More than 10(3.5) PFU of virus was detected in salivary glands of both species of mosquitoes on day 21 of extrinsic incubation. Forty percent (2/5) of Ae. vexans nip. transmitted the virus into serum-agar after ingesting 10(4.3) PFU/ml of virus blood mixture. In experiments with Cx. tritaeniorhynchus ingesting 10(7.5) PFU/ml of virus blood mixture, 57 per cent (4/7) were able to transmit the virus to suckling mice and 59 per cent (10/17) transmitted the virus into serum-agar.
采用膜饲法评估了刺扰伊蚊日本亚种（Ae. vexans nipponii，nip.）和三带喙库蚊（Cx. tritaeniorhynchus）对盖塔病毒的媒介能力。在28℃进行21天的外在潜伏期后，两种蚊子体内的盖塔病毒滴度均很高。喂食后21天，刺扰伊蚊日本亚种在病毒剂量为10(5.3) PFU/ml时的感染率为100%（4/4），三带喙库蚊在类似病毒剂量下的感染率为60%（3/5）。外在潜伏期第21天，两种蚊子的唾液腺中均检测到超过10(3.5) PFU的病毒。刺扰伊蚊日本亚种中有40%（2/5）在摄入10(4.3) PFU/ml的病毒血液混合物后将病毒传播到血清琼脂中。在三带喙库蚊摄入10(7.5) PFU/ml的病毒血液混合物的实验中，57%（4/7）能够将病毒传播给乳鼠，59%（10/17）将病毒传播到血清琼脂中。
