Chou P T, Khan A U
Biochem Biophys Res Commun. 1983 Sep 30;115(3):932-7. doi: 10.1016/s0006-291x(83)80024-2.
L-ascorbic acid quenches singlet (1 delta g) molecular oxygen in aqueous media (pH 6.8 for [1H]H2O and pD 7.2 for [2H]D2O) as measured directly by monitoring (0,0) 1 delta g leads to 3 sigma-g emission at 1.28 micron. Singlet oxygen was generated at room temperature in the solutions via photosensitization of sodium chrysene sulfonate; this sulfonated polycyclic hydrocarbon was synthesized to provide a water soluble chromophore inert to usual dye-ascorbate photobleaching. A marked isotope effect is found; kHQ2O is 3.3 times faster than kDQ2O, suggesting ascorbic acid is chemically quenching singlet oxygen.
在水介质中（对于[¹H]H₂O，pH为6.8；对于[²H]D₂O，pD为7.2），L-抗坏血酸可淬灭单线态（¹Δg）分子氧，这是通过监测（0,0）¹Δg在1.28微米处导致的³Σg发射直接测得的。通过菲磺酸钠的光敏化作用在溶液中于室温下产生单线态氧；合成这种磺化多环烃是为了提供一种对常见染料 - 抗坏血酸光漂白呈惰性的水溶性发色团。发现了显著的同位素效应；kH₂O比kD₂O快3.3倍，这表明抗坏血酸在化学上淬灭单线态氧。
