Ozaki L S, Svec P, Nussenzweig R S, Nussenzweig V, Godson G N
Cell. 1983 Oct;34(3):815-22. doi: 10.1016/0092-8674(83)90538-x.
The gene that codes for the surface antigen of Plasmodium knowlesi sporozoites (CS protein) is unsplit and present in the genome in only one copy. The CS protein, as deduced from DNA sequence analysis of the structural gene, has an unusual structure with the central 40% of the polypeptide chain present as 12 tandemly repeated amino acid peptide units flanked by regions of highly charged amino acids. The protein has an amino-terminal hydrophobic amino acid signal sequence and a hydrophobic carboxy-terminal anchor sequence. The coding sequence of the gene has an AT content of 53%, compared with 70% AT in the 5' and 3' flanking sequences, and is contained entirely within an 11 kb Eco RI genomic DNA fragment. This genomic fragment expresses the CS protein in E. coli, indicating that the parasite promoter and ribosome binding site signals can be recognized in E. coli.
诺氏疟原虫子孢子表面抗原（CS蛋白）的编码基因是不间断的，且在基因组中仅以单拷贝形式存在。从该结构基因的DNA序列分析推断，CS蛋白具有不寻常的结构，多肽链中央40%呈12个串联重复的氨基酸肽单元，两侧为高电荷氨基酸区域。该蛋白具有氨基末端疏水氨基酸信号序列和疏水羧基末端锚定序列。该基因的编码序列AT含量为53%，而5'和3'侧翼序列的AT含量为70%，并且完全包含在一个11kb的Eco RI基因组DNA片段中。这个基因组片段在大肠杆菌中表达CS蛋白，表明寄生虫启动子和核糖体结合位点信号在大肠杆菌中能够被识别。
