Fogelman A M, Seager J, Groopman J E, Berliner J A, Haberland M E, Edwards P A, Golde D W
J Immunol. 1983 Nov;131(5):2368-73.
As little as 1 microliter of serum-free supernatant from Mo(t), an established lymphocyte line, when added to a 500-microliters incubation of macrophages derived from human monocytes, significantly decreased the receptor-mediated uptake and degradation of three cholesterol-rich molecules: low density lipoprotein (LDL); LDL complexed to dextran sulfate; and LDL modified by malondialdehyde (MDA-LDL). In contrast, the receptor-mediated uptake and degradation of mannosyl bovine serum albumin was increased three-fold. The Mo(t) supernatant did not contain competitive inhibitors of the cholesterol-rich ligands, and it did not alter macrophage receptor-independent endocytosis, protein synthesis, or phagocytosis of heat-killed yeast. The effect of the Mo(t) supernatant was specific for macrophages and was abolished by preincubation of the supernatant with trypsin, which indicates that the active substances are protein in nature. The decrease in the uptake and degradation of MDA-LDL induced by preincubating the macrophages with Mo(t) supernatant appeared to result from a decrease in the number of receptors for this ligand at the cell surface. The isolation of these lymphokines should offer new insights into macrophage receptor-mediated endocytosis, and may yield substances useful in preventing foam cell formation in atherosclerosis.
将来自已建立的淋巴细胞系Mo(t)的仅1微升无血清上清液添加到500微升源自人单核细胞的巨噬细胞培养液中时，可显著降低三种富含胆固醇分子的受体介导摄取和降解：低密度脂蛋白（LDL）；与硫酸葡聚糖复合的LDL；以及经丙二醛修饰的LDL（MDA-LDL）。相比之下，甘露糖基牛血清白蛋白的受体介导摄取和降解增加了三倍。Mo(t)上清液不含富含胆固醇配体的竞争性抑制剂，且不改变巨噬细胞非受体依赖性内吞作用、蛋白质合成或热灭活酵母的吞噬作用。Mo(t)上清液的作用对巨噬细胞具有特异性，且通过将上清液与胰蛋白酶预孵育可消除该作用，这表明活性物质本质上是蛋白质。用Mo(t)上清液预孵育巨噬细胞导致MDA-LDL摄取和降解减少，这似乎是由于细胞表面该配体的受体数量减少所致。这些淋巴因子的分离应为深入了解巨噬细胞受体介导的内吞作用提供新的见解，并可能产生有助于预防动脉粥样硬化中泡沫细胞形成的物质。
