Rothermel C D, Rubin B Y, Murray H W
J Immunol. 1983 Nov;131(5):2542-4.
We have demonstrated previously that mitogen-induced lymphokines activate human monocyte-derived macrophages to inhibit the intracellular replication of Chlamydia psittaci. To identify the factor(s) in crude lymphokines responsible for this antimicrobial effect, we tested human Con A-induced lymphokines for interferon activity. We also attempted to neutralize the lymphokines with a monoclonal antibody directed against human gamma-interferon and examined the ability of partially purified human gamma-interferon to induce macrophage antichlamydial activity. The lymphokine-induced antichlamydial effect was measured by the inhibition of chlamydial inclusion formation in Giemsa-stained macrophage cultures. Our lymphokines were found to be rich in gamma-interferon; treatment of cells for 48 hr before infection with lymphokines containing 300 U/ml of interferon resulted in an 89% inhibition of chlamydial growth. This lymphokine effect was completely abolished by monoclonal antibody against human gamma-interferon, but not by antisera against human alpha- or beta-interferons. In addition, partially purified human gamma-interferon alone induced macrophages to restrict chlamydial growth by 95%. We conclude that it is the gamma-interferon present in human Con A-induced lymphokines that activates monocyte-derived macrophages to inhibit chlamydial replication.
我们先前已证明，丝裂原诱导的淋巴因子可激活人单核细胞衍生的巨噬细胞，以抑制鹦鹉热衣原体的细胞内复制。为了确定粗制淋巴因子中负责这种抗菌作用的因子，我们检测了人Con A诱导的淋巴因子的干扰素活性。我们还试图用针对人γ干扰素的单克隆抗体中和淋巴因子，并检测部分纯化的人γ干扰素诱导巨噬细胞抗衣原体活性的能力。通过抑制吉姆萨染色巨噬细胞培养物中的衣原体包涵体形成来测量淋巴因子诱导的抗衣原体作用。我们发现我们的淋巴因子富含γ干扰素；在用含有300 U/ml干扰素的淋巴因子感染前48小时处理细胞，可导致衣原体生长抑制89%。针对人γ干扰素的单克隆抗体可完全消除这种淋巴因子作用，但针对人α或β干扰素的抗血清则不能。此外，单独的部分纯化人γ干扰素可诱导巨噬细胞将衣原体生长限制95%。我们得出结论，是人Con A诱导的淋巴因子中存在的γ干扰素激活了单核细胞衍生的巨噬细胞以抑制衣原体复制。
