Kirk K L, Dawson D C
J Gen Physiol. 1983 Sep;82(3):297-313. doi: 10.1085/jgp.82.3.297.
Treatment of the apical surface of the isolated, ouabain-inhibited turtle colon with the polyene antibiotic amphotericin B permitted the properties of a barium-sensitive potassium conductance in the basolateral membrane to be discerned from the measurements of transepithelial fluxes and electrical currents. Simultaneous measurements of potassium currents and 42K fluxes showed that the movement of potassium was not in accord with simple diffusion. Two other cations, thallium and rubidium, were also permeable and, in addition, exhibited strong interactions with the potassium tracer fluxes. The results indicate that permeant cations exhibit positive coupling, which is consistent with a single-file mechanism of ion translocation through a membrane channel.
用多烯抗生素两性霉素B处理分离的、哇巴因抑制的龟结肠顶端表面，通过跨上皮通量和电流测量，可以辨别基底外侧膜中钡敏感钾电导的特性。同时测量钾电流和42K通量表明，钾的移动不符合简单扩散。另外两种阳离子铊和铷也具有通透性，并且与钾示踪通量表现出强烈的相互作用。结果表明，渗透性阳离子表现出正耦合，这与离子通过膜通道的单排机制一致。
