天然梯度聚丙烯酰胺平板凝胶中二价阳离子依赖性三磷酸腺苷酶的增溶与分离
Solubilization and separation of divalent cation dependent adenosinetriphosphatase in native gradient polyacrylamide slab gel.
作者信息
Nagy A, Simon J
出版信息
J Neurosci Methods. 1983 Sep;9(1):57-64. doi: 10.1016/0165-0270(83)90109-7.
PMID:6314062
Abstract
A new method for the simultaneous solubilization and separation of different cation dependent adenosinetriphosphatases after non-denaturing polyacrylamide gel electrophoresis is described. Using a gradient system, 3 distinct divalent cation dependent adenosinetriphosphatase bands (Mg2+-, Ca2+-, and Mg2+ + Ca2+-dependent) could be separated on the same gel from NP-40 solubilized brain microsomal preparations. This indicates that these 3 adenosinetriphosphatases represent distinct molecular species.
摘要
描述了一种在非变性聚丙烯酰胺凝胶电泳后同时溶解和分离不同阳离子依赖性三磷酸腺苷酶的新方法。使用梯度系统，可以在同一凝胶上从NP-40溶解的脑微粒体制剂中分离出3条不同的二价阳离子依赖性三磷酸腺苷酶带（Mg2 +、Ca2 +和Mg2 + + Ca2 +依赖性）。这表明这3种三磷酸腺苷酶代表不同的分子种类。
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