在体外，纳洛酮可增强促肾上腺皮质激素（ACTH）和血管紧张素II的作用，但不增强钾刺激的醛固酮分泌。

Naloxone potentiates ACTH and angiotension II but not potassium stimulated aldosterone secretion, in vitro.

作者信息

Lymangrover J R, Keku E, Eldridge J C

出版信息

Life Sci. 1983 Oct 17;33(16):1605-12. doi: 10.1016/0024-3205(83)90702-6.

Abstract

The effects of naloxone on basal and ACTH, Angiotensin II (AII) and [K+] o stimulated aldosterone secretion from superfused rat adrenocortical tissue were investigated. A high dose (10(-6) M) of naloxone inhibited while a smaller dose (10(-10) M) potentiated and doses of 10(-8) or 10(-12) M naloxone were without an effect on ACTH stimulated aldosterone secretion. A potentiation of AII stimulated aldosterone secretion was observed beginning 2 hrs after 10(-6) or 10(-10) M naloxone was administered while no effect was observed with 10(-4) M naloxone. No effects of 10(-6), 10(-8), 10(-12) M naloxone were detected on aldosterone secretion stimulated by transiently elevating extracellular potassium. Naloxone from 10(-4) to 10(-12) M did not appear to significantly influence basal steroidogenic activity under these conditions. These findings demonstrate that the "opioid antagonist" naloxone has prominent actions on adrenocortical tissue. Both the specificity and lack of specificity of the action of this agent to influence the activity of the 3 secretagogues suggest that naloxone and possibly a naturally occurring endogenous ligand interacts with one or more membrane receptor distinct from the ACTH receptor. A naturally occurring ligand for this receptor could play a prominent role in the physiological regulation of adrenal steroid secretion.

摘要

研究了纳洛酮对基础状态下以及促肾上腺皮质激素（ACTH）、血管紧张素 II（AII）和高钾（[K⁺]o）刺激的灌流大鼠肾上腺皮质组织醛固酮分泌的影响。高剂量（10⁻⁶ M）的纳洛酮抑制醛固酮分泌，而较小剂量（10⁻¹⁰ M）则增强醛固酮分泌，10⁻⁸ 或 10⁻¹² M 的纳洛酮对 ACTH 刺激的醛固酮分泌无影响。在给予 10⁻⁶ 或 10⁻¹⁰ M 纳洛酮 2 小时后，观察到 AII 刺激的醛固酮分泌增强，而 10⁻⁴ M 纳洛酮则无此作用。未检测到 10⁻⁶、10⁻⁸、10⁻¹² M 纳洛酮对细胞外钾短暂升高刺激的醛固酮分泌有影响。在这些条件下，10⁻⁴ 至 10⁻¹² M 的纳洛酮似乎未显著影响基础类固醇生成活性。这些发现表明，“阿片类拮抗剂”纳洛酮对肾上腺皮质组织有显著作用。该药物影响三种促分泌素活性的作用既有特异性又缺乏特异性，这表明纳洛酮以及可能的天然内源性配体与一种或多种不同于 ACTH 受体的膜受体相互作用。这种受体的天然配体可能在肾上腺类固醇分泌的生理调节中起重要作用。