Tanaka M, Yamamoto T, Sawai T
Mol Gen Genet. 1983;191(3):442-50. doi: 10.1007/BF00425761.
The fine structure of the genes tnpA, tnpR and res of Tn2603 required for its own transposition, was determined. The order of the genes was tnpA-tnpR-res from the right end of the right hand side region in Tn2603, the tnpA and tnpR encoded gene products having molecular weights of 110,000 and 21,000, respectively. The 110,000 molecular weight polypeptides was absolutely required for replicon fusion as the first stage of transposition, and named transposase. On the other hand, the 21,000 molecular weight polypeptide was necessary for resolution of the cointegrate as the second stage of transposition, and named resolvase. We also examined the ability of various transposons, assumed to be closely related, to complement the tnpA and tnpR mutations of Tn2603. The results indicated that the mercury resistance transposon, Tn2613, and Tn501, can complement both genes, but TnAs and gamma delta cannot at all. Tn501 had much less efficiency of complementation for tnpA than Tn2613. We have also discovered that the transposition frequency of transposons in the tn2613 family systematically depend on their size of transposon.
确定了转座子Tn2603自身转座所需的基因tnpA、tnpR和res的精细结构。这些基因的顺序是从Tn2603右手侧区域的右端开始为tnpA - tnpR - res，tnpA和tnpR编码的基因产物分子量分别为110,000和21,000。转座的第一阶段即复制子融合绝对需要分子量为110,000的多肽，并将其命名为转座酶。另一方面，分子量为21,000的多肽是转座第二阶段即共整合体解离所必需的，并将其命名为解离酶。我们还检测了各种假定密切相关的转座子对Tn2603的tnpA和tnpR突变进行互补的能力。结果表明，抗汞转座子Tn2613和Tn501可以对这两个基因进行互补，但TnAs和γδ根本不能。Tn501对tnpA的互补效率比Tn2613低得多。我们还发现，tn2613家族中转座子的转座频率系统地取决于它们的转座子大小。
