tnpA基因的核苷酸序列完善了假单胞菌转座子Tn501的序列。
The nucleotide sequence of the tnpA gene completes the sequence of the Pseudomonas transposon Tn501.
作者信息
Brown N L, Winnie J N, Fritzinger D, Pridmore R D
出版信息
Nucleic Acids Res. 1985 Aug 12;13(15):5657-69. doi: 10.1093/nar/13.15.5657.
Abstract
The nucleotide sequence of the gene (tnpA) which codes for the transposase of transposon Tn501 has been determined. It contains an open reading frame for a polypeptide of Mr = 111,500, which terminates within the inverted repeat sequence of the transposon. The reading frame would be transcribed in the same direction as the mercury-resistance genes and the tnpR gene. The amino acid sequence predicted from this reading frame shows 32% identity with that of the transposase of the related transposon Tn3. The C-terminal regions of these two polypeptides show slightly greater homology than the N-terminal regions when conservative amino acid substitutions are considered. With this sequence determination, the nucleotide sequence of Tn501 is fully defined. The main features of the sequence are briefly presented.
摘要
编码转座子Tn501转座酶的基因（tnpA）的核苷酸序列已被确定。它包含一个编码Mr = 111,500多肽的开放阅读框，该多肽在转座子的反向重复序列内终止。该阅读框将与汞抗性基因和tnpR基因以相同方向转录。从这个阅读框预测的氨基酸序列与相关转座子Tn3的转座酶的氨基酸序列具有32%的同一性。当考虑保守氨基酸取代时，这两种多肽的C末端区域比N末端区域显示出略高的同源性。通过这个序列测定，Tn501的核苷酸序列已被完全确定。简要介绍了该序列的主要特征。
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