Cloning and restriction enzyme analysis of a Neisseria gonorrhoeae plasmid.

A 4.2-kb cryptic plasmid (pDS1) isolated from Neisseria gonorrhoeae FA101 has been cloned in Escherichia coli using the plasmid pUC9. Restriction enzyme maps of both native pDS1 and cloned pDS1 have been constructed; these indicate that the external C may be modified in some of the 5' CCGG 3' sites of the gonococcal plasmid.