在注射mRNA的非洲爪蟾卵母细胞中功能性鸡脑γ-氨基丁酸-苯二氮卓-巴比妥酸盐/受体复合物的合成。

Synthesis of functional chick brain GABA-benzodiazepine-barbiturate/receptor complexes in mRNA-injected Xenopus oocytes.

作者信息

Smart T G, Constanti A, Bilbe G, Brown D A, Barnard E A

出版信息

Neurosci Lett. 1983 Sep 19;40(1):55-9. doi: 10.1016/0304-3940(83)90092-7.

Abstract

Membrane conductance changes evoked by bath-applied GABA (2-640 microM) and some related agonists were recorded intracellularly in Xenopus oocytes previously injected with chick brain mRNA. Muscimol and 3-aminopropanesulphonate were approximately 4 and 0.25 times as potent as GABA in producing a conductance increase. GABA responses were antagonized by bicuculline (10 microM) or picrotoxinin (10-100 microM) but were clearly enhanced by the benzodiazepine (BZ) receptor ligand chlorazepate or by pentobarbitone. We conclude that an appropriate fraction of brain mRNA was capable of directing the synthesis and correct insertion of functional GABA-BZ-barbiturate/receptor complexes in the oocyte membrane.

摘要

在预先注射了鸡脑信使核糖核酸（mRNA）的非洲爪蟾卵母细胞中，通过细胞内记录法记录了由浴槽施加的γ-氨基丁酸（GABA，2 - 640微摩尔）及一些相关激动剂引起的膜电导变化。蝇蕈醇和3-氨基丙烷磺酸盐在产生电导增加方面的效力分别约为GABA的4倍和0.25倍。GABA反应被荷包牡丹碱（10微摩尔）或印防己毒素（10 - 100微摩尔）拮抗，但明显被苯二氮䓬（BZ）受体配体氯氮䓬或戊巴比妥增强。我们得出结论，适当比例的脑mRNA能够指导功能性GABA - BZ - 巴比妥酸盐/受体复合物在卵母细胞膜中的合成和正确插入。