Biosynthesis of the neurofilament heavy subunit in Xenopus oocytes microinjected with rat brain poly(A)+ RNA.

作者信息

Cross D, Allende M L, Krauss R Y, Fuentes M E, Kaltwasser G, Alvarez J, Inestrosa N C

机构信息

Department of Cell Biology, Faculty of Biological Sciences, School of Medicine, Catholic University of Chile, Santiago.

出版信息

Mol Biol Rep. 1987;12(4):265-71. doi: 10.1007/BF00444678.

In order to study the expression of the major subunit of neurofilaments (NFs), rat brain poly(A)+ RNA was purified by three different procedures and was injected in Xenopus laevis oocytes. This system was able to translate efficiently the 200 kDa NF subunit as shown by a dot-blot immunoassay and by immunoprecipitation of labeled NF polypeptides.