Identification and characterization of the glucuronide metabolite of diethyldithiocarbamic acid in the bile from the isolated perfused rat liver by gas chromatography and mass spectrometry.

The glucuronide metabolite of diethyldithiocarbamic acid has been identified in bile from the isolated perfused rat liver. The bile was chromatographed on XAD-2 resin, pertrimethylsilylated with BSTAF + 1% TMCS, identified and characterized by combined gas chromatography and mass spectrometry. The glucuronide characterized as the silylated derivative showed no molecular ion but a small fragment ion at M-15(598).