Lance E A, Rhodes C W, Nakon R
Anal Biochem. 1983 Sep;133(2):492-501. doi: 10.1016/0003-2697(83)90115-x.
Potentiometric, visible, infrared, electron spin, and nuclear magnetic resonance studies of the complexation of N-(2-acetamido)iminodiacetic acid (H2ADA) by Ca(II), Mg(II), Mn(II), Zn(II), Co(II), Ni(II), and Cu(II) are reported. Ca(II) and Mg(II) were found not to form 2:1 ADA2- to M(II) complexes, while Mn(II), Cu(II), Ni(II), Zn(II), and Co(II) did form 2:1 metal chelates at or below physiological pH values. Co(II) and Zn(II), but not Cu(II), were found to induce stepwise deprotonation of the amide groups to form [M(H-1ADA)4-(2)]. Formation (affinity) constants for the various metal complexes are reported, and the probable structures of the various metal chelates in solution are discussed on the basis of various spectral data.
报道了关于Ca(II)、Mg(II)、Mn(II)、Zn(II)、Co(II)、Ni(II)和Cu(II)与N-(2-乙酰氨基)亚氨基二乙酸(H2ADA)络合的电位、可见、红外、电子自旋和核磁共振研究。发现Ca(II)和Mg(II)不形成2:1的ADA2-与M(II)络合物，而Mn(II)、Cu(II)、Ni(II)、Zn(II)和Co(II)在生理pH值或低于生理pH值时确实形成2:1的金属螯合物。发现Co(II)和Zn(II)而非Cu(II)会诱导酰胺基团逐步去质子化以形成[M(H-1ADA)4-(2)]。报道了各种金属络合物的形成（亲和）常数，并根据各种光谱数据讨论了溶液中各种金属螯合物的可能结构。
