Gelb J, Leary J H, Rosenberger J K
Avian Dis. 1983 Jul-Sep;27(3):667-78.
The prevalence of Arkansas (Ark)-type infectious bronchitis virus (IBV) in Delmarva peninsula broiler-type chickens was determined. The immunity of 5-to-11-week-old commercial broilers was evaluated by intraocular inoculation with Ark-type DPI strain (Ark DPI) challenge virus and collection of tracheal swabbings 5 days later. Serum Ark-type antibody titers were obtained using the virus-neutralization test. Eighty-five flocks were tested from January to August 1981. Nearly 60% of the flocks had substantial (greater than or equal to 70%) local immunity of the upper respiratory tract. Twenty-two percent had intermediate (50-69%) and 19% of the flocks had low (less than or equal to 40%) levels of local immunity. Serum antibody titers generally agreed with challenge results. In addition, high Ark-type IBV neutralizing-antibody titers were found in 16 Delmarva broiler breeder flocks. Seven current IBV field isolates were characterized for antigenic similarity to Ark DPI. Four isolates contained Ark antigen(s) based on significant neutralization in virus-neutralization tests and on substantial immunity to challenge afforded by Ark DPI virus immunization. Three isolates did not appear to contain Ark antigen(s). Immunization of chickens with Ark DPI virus afforded substantial protection against Connecticut- and homologous-type virus challenge, partial immunity (63%) against JMK, and no protection against the Massachusetts 41 strain of IBV.
对德尔马瓦半岛肉用型鸡群中阿肯色州（Ark）型传染性支气管炎病毒（IBV）的流行情况进行了测定。通过眼内接种Ark型DPI毒株（Ark DPI）攻毒病毒，并在5天后采集气管拭子，对5至11周龄的商品肉鸡的免疫力进行了评估。使用病毒中和试验获得血清Ark型抗体滴度。1981年1月至8月对85个鸡群进行了检测。近60%的鸡群上呼吸道具有较强（大于或等于70%）的局部免疫力。22%的鸡群免疫力中等（50 - 69%），19%的鸡群局部免疫力较低（小于或等于40%）。血清抗体滴度总体上与攻毒结果相符。此外，在16个德尔马瓦肉种鸡群中发现了高滴度的Ark型IBV中和抗体。对7株当前的IBV野外分离株进行了与Ark DPI抗原相似性的鉴定。基于病毒中和试验中的显著中和作用以及Ark DPI病毒免疫所提供的对攻毒的较强免疫力，4株分离株含有Ark抗原。3株分离株似乎不含有Ark抗原。用Ark DPI病毒免疫鸡可对康涅狄格型和同源型病毒攻毒提供较强保护，对JMK型病毒提供部分免疫力（63%），但对IBV的马萨诸塞41株病毒攻毒无保护作用。
