Kanitakis J, Schmitt D, Bernard A, Boumsell L, Thivolet J
Br J Dermatol. 1983 Nov;109(5):509-13. doi: 10.1111/j.1365-2133.1983.tb07672.x.
Anti-D47 is a monoclonal antibody reacting with a surface antigen of human cortical thymocytes (different from the T6 antigen). It was prepared by immunization of mice with human thymocytes. The known cross-reactivity of monoclonal antibodies prepared against thymic cells with skin components prompted us to test anti-D47 on human skin as well as animal tissues. No labelling was observed on animal tissues. On human skin, anti-D47 was found to react with the secretory portion of eccrine sweat glands (ESG), i.e. with a cytoplasmic antigen of the secretory cells lining the deep portion of ESG glomeruli. No labelling was observed on apocrine sweat glands or on the excretory portion of ESG. Anti-D47 was also tested on histologically proven extramammary (vulvar) Paget's disease and basal cell epitheliomas: no staining of the neoplastic cells was observed. Anti-D47 appears to be an immunological marker of the secretory cells of human ESG and a new tool for the investigation of human sweat gland pathology.
抗 D47 是一种单克隆抗体，可与人皮质胸腺细胞的表面抗原发生反应（不同于 T6 抗原）。它是通过用人胸腺细胞免疫小鼠制备的。针对胸腺细胞制备的单克隆抗体与皮肤成分的已知交叉反应性促使我们在人体皮肤以及动物组织上测试抗 D47。在动物组织上未观察到标记。在人体皮肤上，发现抗 D47 与小汗腺（ESG）的分泌部分发生反应，即与 ESG 肾小球深部内衬的分泌细胞的细胞质抗原发生反应。在顶泌汗腺或 ESG 的排泄部分未观察到标记。还对经组织学证实的乳腺外（外阴）佩吉特病和基底细胞上皮瘤进行了抗 D47 测试：未观察到肿瘤细胞染色。抗 D47 似乎是人 ESG 分泌细胞的免疫标志物，也是研究人体汗腺病理学的新工具。
