Amiot M, Dastot H, Fabbi M, Degos L, Bernard A, Boumsell L
INSERM U93, Institut de Recherche sur les Maladies du Sang, Paris, France.
Immunogenetics. 1988;27(3):187-95. doi: 10.1007/BF00346585.
The first cluster of differentiation (CD1) defines at least three distinct human thymic cell-surface differentiation antigens-CD1a, CD1b, and CD1c. We looked for structural homology of the three CD1 heavy chains at their peptide level by two-dimensional peptide maps. We show here that the CD1a Mr 49,000 heavy chain and the CD1b Mr 45,000 heavy chain appear to be more homologous to each other than to the CD1c Mr 43,000 heavy chain and that only one tyrosil peptide is common to the three heavy chains. Study of the CD1 heavy chains from several individuals reveals a very limited polymorphism of these molecules. We also demonstrate here that CD1a or CD1a-like molecules and other CD1 molecules can form intermolecular complexes on the surface of normal thymus cells. Molecules that are structurally very similar to CD1a molecules are associated noncovalently either with CD1c molecules or with CD1b molecules, and only CD1a molecules can associate covalently with CD8 molecules. In contrast, we could not find these intermolecular complexes on the surface of leukemic T-cell lines in culture.
第一分化簇（CD1）定义了至少三种不同的人类胸腺细胞表面分化抗原——CD1a、CD1b和CD1c。我们通过二维肽图在肽水平上寻找这三种CD1重链的结构同源性。我们在此表明，49,000分子量的CD1a重链和45,000分子量的CD1b重链彼此之间似乎比与43,000分子量的CD1c重链更同源，并且这三条重链只有一个酪氨酸肽是共同的。对来自多个个体的CD1重链的研究揭示了这些分子的多态性非常有限。我们在此还证明，CD1a或CD1a样分子与其他CD1分子可在正常胸腺细胞表面形成分子间复合物。在结构上与CD1a分子非常相似的分子非共价地与CD1c分子或CD1b分子相关联，并且只有CD1a分子可与CD8分子共价结合。相比之下，我们在培养的白血病T细胞系表面未发现这些分子间复合物。
