[使用十二烷基硫酸钠和皂角苷去污剂用于绵羊肾脏中（钠+钾）ATP酶的展示与纯化]

[Use of SDS and saponin detergents for the demonstration and purification of (Na+K+)ATPase in sheep kidney].

作者信息

Foussard-Guilbert F, Ermias A, Laget P, Tanguy G, Girault M, Jallet P

出版信息

Biochimie. 1983 Aug-Sep;65(8-9):519-27. doi: 10.1016/s0300-9084(83)80134-5.

Abstract

Incubation of membrane fragments from sheep kidney outer medulla in detergents such as SDS or saponin revealed increased specific (NA+) ATPase activity. Measurement of enzyme phosphorylation from [gamma 32P] ATP showed irreversible alteration of EP to (Na+) ATPase activity ratio when SDS is used as unmasking agent. These results were found with unmasked and purified enzyme.

摘要

用十二烷基硫酸钠（SDS）或皂角苷等去污剂孵育绵羊肾外髓质的膜片段，发现其特异性（钠）ATP酶活性增加。用[γ-32P]ATP测量酶磷酸化时发现，当使用SDS作为去掩盖剂时，磷酸化酶（EP）与（钠）ATP酶活性的比值发生了不可逆的改变。未掩盖和纯化的酶均得到了这些结果。