Shemanova G F, Trapezov E V, Musina E E, Vlasova E V, Panteleev E I
Biull Eksp Biol Med. 1983 Nov;96(11):82-4.
The methods for determining the level of type A Perfringens antitoxin in human blood sera were examined and compared. The ratios for correlating the data obtained in the toxin neutralization test (NT) in vivo, in the passive hemagglutination test (PHT), and as a result of the enzyme-labeled immunosorbent assay (ELISA) with regard to the antitoxin level measured in the NT in vitro were equal to 0.88, 0.64 and 0.39, respectively. The sensitivity of the NT in vivo and in vitro was 0.25 IU/ml, that of the PHT 0.01-0.005 IU/ml, and that of the ELISA 0.01-0.02 IU/ml Perfringens antitoxin. To perform the NT, not less than 1 ml blood serum is required, while for the PHT and ELISA, 0.1-0.05 ml. Provided hyghly purified anatoxin is used for preparing the erythrocyte diagnosticum Perfringens, and polysterene plates are sensitized in performing the ELISA, all the reactions are specific. While titrating human blood sera containing type A Perfringens antitoxin, use in the PHT may be made of type A Perfringens rabbit antiserum as reference.
对人血清中A型产气荚膜梭菌抗毒素水平的测定方法进行了研究和比较。体内毒素中和试验（NT）、被动血凝试验（PHT）以及酶联免疫吸附测定（ELISA）所获得的数据与体外NT中测得的抗毒素水平的相关比率分别为0.88、0.64和0.39。体内和体外NT的灵敏度为0.25 IU/ml，PHT的灵敏度为0.01 - 0.005 IU/ml，ELISA的灵敏度为0.01 - 0.02 IU/ml产气荚膜梭菌抗毒素。进行NT时，需要不少于1 ml血清，而进行PHT和ELISA时，需要0.1 - 0.05 ml。如果使用高度纯化的类毒素来制备产气荚膜梭菌红细胞诊断制剂，并且在进行ELISA时对聚苯乙烯板进行致敏处理，那么所有反应都是特异性的。在滴定含A型产气荚膜梭菌抗毒素的人血清时，PHT中可使用A型产气荚膜梭菌兔抗血清作为对照。
