Thesleff I, Ekblom P, Keski-Oja J
Cancer Res. 1983 Dec;43(12 Pt 1):5902-9.
Sarcoma growth factor (SGF) induces proliferation and anchorage-independent growth of nonmalignant cells. It competes with epidermal growth factor (EGF) for the EGF-receptors at the cell surface. SGF-like factors have recently been isolated from embryos, suggesting that SGFs may represent embryonic forms of EGF. Therefore, we have tested whether SGF preparations affect organogenesis and differentiation of cultured embryonic tissues. The embryonic tooth rudiments were cultivated in the presence of SGF and EGF. Stimulation of vascularization was seen in both of these organ cultures. Therefore, we propose that endothelial cells may be target cells for SGF, and SGF may be involved in the control of vascularization during embryogenesis. SGF and also, to a certain extent, EGF profoundly inhibited morphogenesis and differentiation of the tooth germ, with concomitant stimulation of vascularization. Analysis of cell proliferation revealed that some cell types of the tooth germ did not respond to SGF by proliferation, while a stimulation by EGF was observed. Nevertheless, tooth morphogenesis was also slightly inhibited by EGF, suggesting that growth factors which enhance proliferation do not necessarily stimulate morphogenesis and differentiation. Since the SGF preparations contain several factors, the effects observed could be due to the action of one or more factors.
肉瘤生长因子（SGF）可诱导非恶性细胞增殖并使其在不依赖贴壁的情况下生长。它在细胞表面与表皮生长因子（EGF）竞争EGF受体。最近已从胚胎中分离出SGF样因子，这表明SGF可能代表EGF的胚胎形式。因此，我们测试了SGF制剂是否会影响培养的胚胎组织的器官发生和分化。将胚胎牙胚在SGF和EGF存在的情况下进行培养。在这两种器官培养物中均观察到血管生成受到刺激。因此，我们提出内皮细胞可能是SGF的靶细胞，并且SGF可能参与胚胎发育过程中血管生成的调控。SGF以及在一定程度上EGF均能显著抑制牙胚的形态发生和分化，同时刺激血管生成。细胞增殖分析显示，牙胚的某些细胞类型对SGF的增殖无反应，而观察到EGF能刺激其增殖。然而，EGF也会轻微抑制牙齿形态发生，这表明促进增殖的生长因子不一定会刺激形态发生和分化。由于SGF制剂包含多种因子，观察到的效应可能是由于一种或多种因子的作用。
