Fyhrquist F, Grönhagen-Riska C, Hortling L, Forslund T, Tikkanen I, Klockars M
Clin Exp Hypertens A. 1983;5(7-8):1319-30. doi: 10.3109/10641968309048860.
The inhibitors of angiotensin converting enzyme (ACE), captopril and enalapril, were found to increase ACE concentration in cultured human endothelial cells from cord artery as measured with a novel ACE assay employing MK 351A, an inhibitor of ACE, and with immunofluorescense labeling using anti-human lung ACE antibody. Dexamethasone (10 nM) also increased ACE and potentiated the increase of cellular ACE caused by captopril. Similar effects of ACE inhibitors were seen in cultured human macrophages, particularly after prestimulation with E. coli lipopolysaccharide. In Wistar Kyoto rats, captopril caused a 3-fold increase of serum ACE, while dexamethasone (40 ug/day, 14 days) did not increase serum ACE. Combined treatment with captopril and dexamethasone caused a 5-fold increase of ACE in purified lung plasma membranes. ACE inhibitors induce increased ACE biosynthesis in endothelial cells, and in macrophages. The rise of cellular ACE with ACE inhibitors is potentiated by glucocorticoid.
血管紧张素转换酶（ACE）抑制剂卡托普利和依那普利，在用一种采用ACE抑制剂MK 351A的新型ACE测定法以及使用抗人肺ACE抗体的免疫荧光标记法测量时，被发现会增加来自脐动脉的培养人内皮细胞中的ACE浓度。地塞米松（10 nM）也会增加ACE，并增强卡托普利引起的细胞ACE增加。在培养的人巨噬细胞中也观察到了ACE抑制剂的类似作用，特别是在用大肠杆菌脂多糖预刺激后。在Wistar Kyoto大鼠中，卡托普利使血清ACE增加了3倍，而地塞米松（40 μg/天，14天）并未增加血清ACE。卡托普利和地塞米松联合治疗使纯化的肺质膜中的ACE增加了5倍。ACE抑制剂可诱导内皮细胞和巨噬细胞中ACE生物合成增加。糖皮质激素可增强ACE抑制剂导致的细胞ACE升高。
