Rana catesbeiana tail fin lactogenic hormone receptor: hydrodynamic characterization.

The hydrodynamic properties of the 125I-hGH-receptor-detergent complex of the tadpole tail fin were determined. Based on the distribution coefficient from chromatography on Sepharose 6B the Stokes' radius of the complex was 5.03 nm. Using centrifugation through sucrose/H2O and sucrose/2H2O gradients the complex was determined to have a sedimentation coefficient of 4.05 S and a partial specific volume of 0.810 cm3/g. The 125I-hGH-receptor-detergent complex was calculated to have a mol wt of 121,000 and a frictional ratio of 1.38. Based on a calculated detergent content of 0.430 g/g complex the mol weight of the 125I-hGH-receptor was 69,100 and the mol wt of the receptor alone was 47,100.