Jaffe R C
Comp Biochem Physiol B. 1983;76(3):529-33. doi: 10.1016/0305-0491(83)90287-0.
The hydrodynamic properties of the 125I-hGH-receptor-detergent complex of the tadpole tail fin were determined. Based on the distribution coefficient from chromatography on Sepharose 6B the Stokes' radius of the complex was 5.03 nm. Using centrifugation through sucrose/H2O and sucrose/2H2O gradients the complex was determined to have a sedimentation coefficient of 4.05 S and a partial specific volume of 0.810 cm3/g. The 125I-hGH-receptor-detergent complex was calculated to have a mol wt of 121,000 and a frictional ratio of 1.38. Based on a calculated detergent content of 0.430 g/g complex the mol weight of the 125I-hGH-receptor was 69,100 and the mol wt of the receptor alone was 47,100.
测定了蝌蚪尾鳍125I - 人生长激素受体 - 去污剂复合物的流体动力学性质。根据在琼脂糖6B上的色谱分配系数，复合物的斯托克斯半径为5.03纳米。通过蔗糖/H₂O和蔗糖/²H₂O梯度离心，确定复合物的沉降系数为4.05 S，比容为0.810 cm³/g。计算得出125I - 人生长激素受体 - 去污剂复合物的分子量为121,000，摩擦比为1.38。基于计算得出的复合物中去污剂含量为0.430 g/g，125I - 人生长激素受体的分子量为69,100，单独受体的分子量为47,100。
