细胞色素氧化酶对氧的激活作用：通过流动闪光观察到的一种新的光谱中间体。
Oxygen activation by cytochrome oxidase: a new spectral intermediate observed by flow-flash.
作者信息
Brunori M, Gibson Q H
出版信息
EMBO J. 1983;2(11):2025-6. doi: 10.1002/j.1460-2075.1983.tb01695.x.
Abstract
The reaction of cytochrome-c-oxidase with O2 has been reinvestigated at 2 degrees C by a flow laser pulsed method. The experiments indicate that a new spectral intermediate, populated with a rate constant of approximately 5000/s, is observed in a wavelength range in which heme a is the only chromophore (445 and 430 nm). The results are interpreted with reference to previous data obtained in the near infrared region, and it is suggested that breakage of the (enzyme bound) dioxygen bond is associated to a spectral perturbation of the cytochrome a3-CuB binuclear center.
摘要
通过流动激光脉冲法在2℃下对细胞色素c氧化酶与O2的反应进行了重新研究。实验表明，在血红素a是唯一发色团的波长范围内（445和430nm）观察到一种新的光谱中间体，其生成速率常数约为5000/s。参考先前在近红外区域获得的数据对结果进行了解释，并表明（酶结合的）双氧键的断裂与细胞色素a3-CuB双核中心的光谱扰动有关。
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本文引用的文献
1
KINETIC OBSERVATIONS ON THE NEAR INFRARED BAND OF CYTOCHROME C OXIDASE.细胞色素C氧化酶近红外波段的动力学观测
J Biol Chem. 1965 Jun;240:2694-8.
2
Studies on cytochrome oxidase. I. Absolute and difference absorption spectra.细胞色素氧化酶的研究。I. 绝对吸收光谱和差示吸收光谱。
J Biol Chem. 1960 Mar;235:845-52.
3
Reaction of oxygen with cytochrome c oxidase from Paracoccus denitrificans.氧气与反硝化副球菌细胞色素c氧化酶的反应。
J Biol Chem. 1981 Oct 10;256(19):10092-8.
4
Structural features and the reaction mechanism of cytochrome oxidase: iron and copper X-ray absorption fine structure.细胞色素氧化酶的结构特征与反应机制：铁和铜的X射线吸收精细结构
Biophys J. 1981 Jun;34(3):465-98. doi: 10.1016/S0006-3495(81)84863-1.
5
Characterization of the low-temperature intermediates of the reaction of fully reduced soluble cytochrome oxidase with oxygen by electron-paramagnetic-resonance and optical spectroscopy.通过电子顺磁共振和光谱学对完全还原的可溶性细胞色素氧化酶与氧气反应的低温中间体进行表征。
Biochem J. 1980 Jan 1;185(1):139-54. doi: 10.1042/bj1850139.
6
Studies on partially reduced mammalian cytochrome oxidase. Reactions with carbon monoxide and oxygen.部分还原的哺乳动物细胞色素氧化酶的研究。与一氧化碳和氧气的反应。
Biochem J. 1974 Feb;137(2):205-15. doi: 10.1042/bj1370205.
7
The reaction of reduced cytochrome C oxidase with oxygen.还原型细胞色素C氧化酶与氧气的反应。
J Biol Chem. 1967 Apr 25;242(8):1782-7.
8
Functional intermediates in the reaction of membrane-bound cytochrome oxidase with oxygen.膜结合细胞色素氧化酶与氧气反应中的功能中间体。
J Biol Chem. 1975 Dec 25;250(24):9226-37.
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