Kmieć Z, Myśliwski A
Exp Gerontol. 1983;18(3):173-84. doi: 10.1016/0531-5565(83)90030-x.
Effects of glucagon or catecholamine stimulation on glucose synthesis from lactate/pyruvate, alanine, fructose or glycerol in isolated hepatocytes from young and old rats were compared. It was found that production of glucose by non-stimulated hepatocytes was almost the same in hepatocytes both from young and old rats. The concentrations of hormones which caused the maximal effect were the same for both compared groups of rats. However, the effect of stimulation by glucagon was significantly greater in hepatocytes of young rats than of old ones, when lactate/pyruvate or alanine were used as substrates, while no such difference was observed when fructose or glycerol were used. Epinephrine and norepinephrine stimulated glucose synthesis from all substrates investigated to the same extent in hepatocytes isolated from both compared groups of rats. The biochemical and physiological aspects of these findings are discussed.
比较了胰高血糖素或儿茶酚胺刺激对年轻和老年大鼠分离肝细胞中乳酸/丙酮酸、丙氨酸、果糖或甘油生成葡萄糖的影响。发现未受刺激的肝细胞产生葡萄糖的情况在年轻和老年大鼠的肝细胞中几乎相同。两组大鼠引起最大效应的激素浓度相同。然而，当使用乳酸/丙酮酸或丙氨酸作为底物时，胰高血糖素刺激对年轻大鼠肝细胞的作用明显大于老年大鼠肝细胞，而使用果糖或甘油时未观察到这种差异。肾上腺素和去甲肾上腺素对两组大鼠分离肝细胞中所有研究底物生成葡萄糖的刺激程度相同。讨论了这些发现的生化和生理方面。
