Mita H, Yui Y, Shida T
Int Arch Allergy Appl Immunol. 1983;72(4):373-6. doi: 10.1159/000234900.
After guinea pigs were exposed to histamine or acetylcholine aerosol for 1 week, alpha 1- and beta-adrenergic and cholinergic muscarinic receptors on the lung membranes were measured by direct binding techniques using 3H-prazosin, l-3H-dihydroalprenolol, and l-3H-quinuclidinyl benzilate, respectively, and adenylate cyclase responses to l-isoproterenol (10(-5) to 10(-8) M) and NaF (20 mM) were also examined on the membranes. After the inhalation, the number of beta-adrenergic receptors was decreased by about 25% without a significant change in binding affinity for l-3H-dihydroalprenolol, while the number and the affinity of alpha 1-adrenergic and muscarinic receptors on the membranes were not significantly different from those in the control animals. When compared with the control animals, the lung membranes showed a reduced adenylate cyclase response to l-isoproterenol, and the response to 10(-5) M of l-isoproterenol was significantly decreased by 11% in the lung membranes. The histamine inhalation showed no significant effect on basal adenylate cyclase activity and adenylate cyclase response to NaF.
将豚鼠暴露于组胺或乙酰胆碱气雾剂中1周后，分别使用³H-哌唑嗪、¹-³H-二氢阿普洛尔和¹-³H-喹核醇基苯甲酸酯通过直接结合技术测量肺膜上的α₁-和β-肾上腺素能及胆碱能毒蕈碱受体，并且还在膜上检测了对l-异丙肾上腺素（10⁻⁵至10⁻⁸M）和氟化钠（20 mM）的腺苷酸环化酶反应。吸入后，β-肾上腺素能受体数量减少约25%，而对¹-³H-二氢阿普洛尔的结合亲和力无显著变化，同时膜上α₁-肾上腺素能和毒蕈碱受体的数量及亲和力与对照动物相比无显著差异。与对照动物相比，肺膜对l-异丙肾上腺素的腺苷酸环化酶反应降低，肺膜中对10⁻⁵M的l-异丙肾上腺素的反应显著降低了11%。吸入组胺对基础腺苷酸环化酶活性和对氟化钠的腺苷酸环化酶反应无显著影响。
