Williams M V, Denekamp J
Int J Radiat Oncol Biol Phys. 1983 Nov;9(11):1731-6. doi: 10.1016/0360-3016(83)90427-3.
The radiation response of the mouse kidney has been assayed after a range of X ray doses given with or without the nitroimidazole misonidazole or the aminothiol WR-2721. Sensitization and protection of the kidney were investigated by comparing the X ray dose needed to achieve a particular level of injury in the presence or absence of the drug. Two functional assays and kidney weight at sacrifice were used to obtain dose response curves. Urine output and 51Chromium EDTA excretion were used as functional assays at 25 and 49 weeks after irradiation. They demonstrated no radio-sensitization by misonidazole with 1, 2 or 5 fractions of X rays. Significant radioprotection was seen when 400 mg kg 1 WR-2721 was given before single X ray doses (PF = 1.34). Similar radioprotection was observed when renal weight at 1 year after irradiation was used as the third assay of damage. These results confirm that the kidney responds as a well-oxygenated normal tissue with only a small protection being afforded against radiation injury by WR-2721.
在给予一系列X射线剂量后，对小鼠肾脏的辐射反应进行了测定，给药时或未给药时分别使用了硝基咪唑类药物米索硝唑或氨基硫醇WR-2721。通过比较在有或没有药物存在的情况下达到特定损伤水平所需的X射线剂量，研究了肾脏的增敏和保护作用。使用两种功能测定方法以及处死时的肾脏重量来获得剂量反应曲线。在照射后25周和49周，将尿量和51铬依地酸排泄用作功能测定方法。结果表明，米索硝唑对单次、两次或五次X射线照射均无放射增敏作用。当在单次X射线剂量前给予400 mg/kg 1的WR-2721时，观察到显著的放射保护作用（防护因子=1.34）。当将照射后1年时的肾脏重量用作第三种损伤测定方法时，观察到了类似的放射保护作用。这些结果证实，肾脏作为一个富氧的正常组织对辐射有反应，WR-2721对辐射损伤仅提供了很小的保护作用。
