Quinn M R, Somerville R A
J Neurochem. 1984 Jan;42(1):290-3. doi: 10.1111/j.1471-4159.1984.tb09733.x.
Scrapie is a transmissible disease that results in progressive degeneration of the central nervous system and death. Although scrapie has been studied histopathologically, relatively little is known concerning neurotransmitter alterations. Specific [3H]muscimol binding to whole brain crude synaptic membranes (CSM) from mice clinically affected with scrapie was significantly (p less than 0.01) reduced, to approximately 73% of that of the controls. Of the brain regions examined, binding to only cerebral CSM was significantly (p less than 0.0001) decreased. Scatchard analyses of saturation curves revealed that the high-affinity (KD = 8 +/- 3 nM) site for muscimol was abolished in cerebral CSM from scrapie-infected mice, while the low-affinity site was unaffected. Binding of [3H]flunitrazepam to cerebral CSM was unaffected by scrapie and was stimulated by GABA to the same extent in both scrapie and control mice. These results suggest that scrapie agent 139A in C57BL/6J mice manifests a portion of its CNS pathology via a high-affinity GABA binding site that is unassociated with the benzodiazepine receptor.
羊瘙痒症是一种可传播的疾病，会导致中枢神经系统进行性退化并最终死亡。尽管已经对羊瘙痒症进行了组织病理学研究，但关于神经递质改变的了解相对较少。与临床感染羊瘙痒症的小鼠全脑粗制突触膜（CSM）的特异性[3H]蝇蕈醇结合显著（p<0.01）降低，降至对照组的约73%。在所检查的脑区中，仅与大脑CSM的结合显著（p<0.0001）降低。对饱和曲线的Scatchard分析表明，感染羊瘙痒症小鼠大脑CSM中蝇蕈醇的高亲和力（KD = 8 +/- 3 nM）位点消失，而低亲和力位点未受影响。[3H]氟硝西泮与大脑CSM的结合不受羊瘙痒症影响，并且在羊瘙痒症小鼠和对照小鼠中受GABA刺激的程度相同。这些结果表明，C57BL/6J小鼠中的羊瘙痒症病原体139A通过与苯二氮䓬受体无关的高亲和力GABA结合位点表现出其部分中枢神经系统病理学特征。
