Shingai R, Christensen B N
Neuroscience. 1983 Nov;10(3):893-7. doi: 10.1016/0306-4522(83)90227-0.
A low resistance suction microelectrode was used to record intracellularly from enzymatically dissociated horizontal cells obtained from catfish retina (Ictalurus punctatus). This microelectrode was connected to a voltage clamp circuit and the transmembrane currents were recorded during depolarizing and hyperpolarizing clamp pulses. Two fast transient inward currents were recorded, one sensitive to tetrodotoxin and one insensitive to this drug. A slower and persistent tetrodotoxin insensitive inward current was also recorded. The results indicate that separate membrane conductances exist for both sodium and calcium ions. We suggest that the action potential recorded from isolated horizontal cells is generated by both sodium and calcium currents.
使用低电阻吸力微电极对从鲶鱼视网膜（斑点叉尾鮰）获取的经酶解的水平细胞进行细胞内记录。该微电极连接到电压钳电路，并在去极化和超极化钳制脉冲期间记录跨膜电流。记录到两种快速瞬态内向电流，一种对河豚毒素敏感，另一种对该药物不敏感。还记录到一种较慢且持续的对河豚毒素不敏感的内向电流。结果表明，钠离子和钙离子存在各自独立的膜电导。我们认为，从分离的水平细胞记录到的动作电位是由钠电流和钙电流共同产生的。
