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由羧基末端修饰的末端蛋白启动噬菌体φ29 DNA复制。

Initiation of phage phi 29 DNA replication by the terminal protein modified at the carboxyl end.

作者信息

Mellado R P, Salas M

出版信息

Nucleic Acids Res. 1983 Nov 11;11(21):7397-407. doi: 10.1093/nar/11.21.7397.

DOI:10.1093/nar/11.21.7397
PMID:6316260
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC326491/
Abstract

A mutant at the carboxyl end of the terminal protein, p3, of phage phi 29 DNA has been constructed by inserting an containing the stop translation codon TGA in the three possible reading frames, immediately downstream of a phage phi 29 DNA fragment coding for all but the last five amino acids of protein p3. The activity in the formation of the p3-dAMP initiation complex in vitro of this mutant as well as another one previously isolated, also mutated at the carboxyl end, have been tested. The results obtained suggest that an intact carboxyl end in the phage phi 29 terminal protein is essential for its normal primer function in DNA replication.

摘要

通过在噬菌体φ29 DNA片段（编码除p3蛋白最后五个氨基酸外的所有氨基酸）的下游，以三种可能的阅读框插入包含终止翻译密码子TGA的片段，构建了噬菌体φ29 DNA末端蛋白p3羧基端的一个突变体。已经测试了该突变体以及先前分离的另一个在羧基端也发生突变的突变体在体外形成p3-dAMP起始复合物的活性。获得的结果表明，噬菌体φ29末端蛋白完整的羧基端对于其在DNA复制中的正常引物功能至关重要。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57c4/326491/3f58f8c5db29/nar00366-0154-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57c4/326491/8647d56184fe/nar00366-0151-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57c4/326491/3f58f8c5db29/nar00366-0154-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57c4/326491/8647d56184fe/nar00366-0151-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57c4/326491/3f58f8c5db29/nar00366-0154-a.jpg

相似文献

1
Initiation of phage phi 29 DNA replication by the terminal protein modified at the carboxyl end.由羧基末端修饰的末端蛋白启动噬菌体φ29 DNA复制。
Nucleic Acids Res. 1983 Nov 11;11(21):7397-407. doi: 10.1093/nar/11.21.7397.
2
Template requirements for initiation of phage phi 29 DNA replication in vitro.体外启动噬菌体φ29 DNA复制的模板要求。
Proc Natl Acad Sci U S A. 1984 Jan;81(1):80-4. doi: 10.1073/pnas.81.1.80.
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Replication of phage phi 29 DNA in vitro: role of the viral protein p6 in initiation and elongation.噬菌体φ29 DNA的体外复制：病毒蛋白p6在起始和延伸过程中的作用
Nucleic Acids Res. 1986 Jun 25;14(12):4923-37. doi: 10.1093/nar/14.12.4923.
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Initiation of phage phi 29 DNA replication in vitro: formation of a covalent complex between the terminal protein, p3, and 5'-dAMP.噬菌体φ29 DNA体外复制的起始：末端蛋白p3与5'-dAMP之间共价复合物的形成。
Proc Natl Acad Sci U S A. 1982 Sep;79(18):5522-6. doi: 10.1073/pnas.79.18.5522.
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Cloning and expression of gene 2, required for the protein-primed initiation of the Bacillus subtilis phage phi 29 DNA replication.枯草芽孢杆菌噬菌体phi 29 DNA复制蛋白引发起始所必需的基因2的克隆与表达。
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High level synthesis in Escherichia coli of the Bacillus subtilis phage phi 29 proteins p3 and p4 under the control of phage lambda PL promoter.在噬菌体λ PL 启动子的控制下，枯草芽孢杆菌噬菌体 phi 29 蛋白 p3 和 p4 在大肠杆菌中的高水平合成。
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Antibodies specific for the phi 29 terminal protein inhibit the initiation of DNA replication in vitro.
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Location of the serine residue involved in the linkage between the terminal protein and the DNA of phage phi 29.
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Purification in an active form of the phage phi 29 protein p4 that controls the viral late transcription.以活性形式纯化控制病毒晚期转录的噬菌体φ29蛋白p4。
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本文引用的文献

1
DNA replication of bacteriophage phi 29: characterization of the intermediates and location of the termini of replication.噬菌体φ29的DNA复制：复制中间体的特性及复制末端的定位
Virology. 1980 Jul 30;104(2):323-38. doi: 10.1016/0042-6822(80)90337-2.
2
Initiation of phage phi 29 DNA replication in vitro: formation of a covalent complex between the terminal protein, p3, and 5'-dAMP.噬菌体φ29 DNA体外复制的起始：末端蛋白p3与5'-dAMP之间共价复合物的形成。
Proc Natl Acad Sci U S A. 1982 Sep;79(18):5522-6. doi: 10.1073/pnas.79.18.5522.
3
Nucleotide sequence of the major early region of bacteriophage phi 29.
噬菌体φ29主要早期区域的核苷酸序列。
Gene. 1982 Mar;17(3):323-35. doi: 10.1016/0378-1119(82)90149-4.
4
In vitro complex formation between bacteriophage phi 29 terminal protein and deoxynucleotide.噬菌体 phi 29 末端蛋白与脱氧核苷酸之间的体外复合物形成
Biochem Biophys Res Commun. 1982 Apr 14;105(3):1031-6. doi: 10.1016/0006-291x(82)91073-7.
5
Structure of protein-containing replicative intermediates of Bacillus subtilis phage phi 29 DNA.枯草芽孢杆菌噬菌体φ29 DNA含蛋白质复制中间体的结构
Virology. 1982 Jan 15;116(1):1-18. doi: 10.1016/0042-6822(82)90398-1.
6
Protein p3 is linked to the DNA of phage phi 29 through a phosphoester bond between serine and 5'-dAMP.蛋白质p3通过丝氨酸与5'-二磷酸腺苷之间的磷酸酯键与噬菌体φ29的DNA相连。
Proc Natl Acad Sci U S A. 1980 Nov;77(11):6425-8. doi: 10.1073/pnas.77.11.6425.
7
The protein covalently linked to the 5' termini of the DNA of Bacillus subtilis phage phi 29 is involved in the initiation of DNA replication.与枯草芽孢杆菌噬菌体phi 29的DNA 5'末端共价连接的蛋白质参与DNA复制的起始。
Virology. 1980 Jul 15;104(1):84-96. doi: 10.1016/0042-6822(80)90367-0.
8
Structure of replicating DNA molecules of Bacillus subtilis bacteriophage phi 29.枯草芽孢杆菌噬菌体 phi 29 复制性 DNA 分子的结构
J Virol. 1980 Apr;34(1):187-99. doi: 10.1128/JVI.34.1.187-199.1980.
9
Factors involved in the initiation of phage phi 29 DNA replication in vitro: requirement of the gene 2 product for the formation of the protein p3-dAMP complex.体外噬菌体φ29 DNA复制起始所涉及的因素：基因2产物对蛋白质p3-dAMP复合物形成的需求。
Nucleic Acids Res. 1983 Mar 11;11(5):1309-23. doi: 10.1093/nar/11.5.1309.
10
Cloning and expression in Escherichia coli of the gene coding for the protein linked to the ends of Bacillus subtilis phage phi 29 DNA.枯草芽孢杆菌噬菌体 phi 29 DNA 末端连接蛋白编码基因在大肠杆菌中的克隆与表达。
Gene. 1983 Jan-Feb;21(1-2):65-76. doi: 10.1016/0378-1119(83)90148-8.