腺病毒5型转化的人细胞系293中病毒DNA序列的整合模式

The pattern of integration of viral DNA sequences in the adenovirus 5-transformed human cell line 293.

作者信息

Spector D J

出版信息

Virology. 1983 Oct 30;130(2):533-8. doi: 10.1016/0042-6822(83)90107-1.

Abstract

The pattern of integrated adenovirus 5 DNA in the adenovirus 5-transformed human cell line 293 was analyzed by DNA blot hybridization. In contrast to a previous report, only sequences from the left end of the genome were detected. The viral DNA was contained in a unique DNA fragment generated by cleavage of 293 DNA with either EcoRI or BamHI, two enzymes which do not cut the integrated viral DNA. Further mapping studies indicated that the integrated sequence was probably colinear with viral DNA. The joining to host cell sequences occurred between viral nucleotide base pairs 1 and 270 (a site for BalI) on one end and 4123 (SmaI site) and 5372 (BalI site) at the other end. The viral DNA was not tandemly repeated. These results suggest that integration of viral sequences into the host genome probably occurred at a single site. If any subsequent duplications of viral DNA took place, then duplication of extensive cellular sequences also must have occurred.

摘要

通过DNA印迹杂交分析了腺病毒5转化的人细胞系293中整合的腺病毒5 DNA的模式。与先前的报告相反，仅检测到基因组左端的序列。病毒DNA包含在通过用EcoRI或BamHI切割293 DNA产生的独特DNA片段中，这两种酶不会切割整合的病毒DNA。进一步的图谱研究表明，整合序列可能与病毒DNA共线。与宿主细胞序列的连接发生在一端的病毒核苷酸碱基对1和270（BalI位点）之间，另一端的4123（SmaI位点）和5372（BalI位点）之间。病毒DNA不是串联重复的。这些结果表明，病毒序列整合到宿主基因组中可能发生在单个位点。如果随后发生了病毒DNA的任何重复，那么广泛的细胞序列重复也必定发生了。