某些单价、二价和三价金属阳离子对大鼠脑内钾离子刺激的对硝基苯磷酸酶的影响。

Effects of certain mono-, di- and trivalent metal cations on the K+-stimulated p-Nitrophenyl phosphatase in rat brain.

作者信息

Bansal S K, Murthy R C, Chandra S V

出版信息

Acta Pharmacol Toxicol (Copenh). 1983 Oct;53(4):333-6. doi: 10.1111/j.1600-0773.1983.tb03431.x.

DOI:10.1111/j.1600-0773.1983.tb03431.x

Abstract

The effects of certain monovalent (Ag+1 and Li+1), divalent (Hg+2, Cu+2, Zn+2, Co+2, Fe+2, Pb+2, Mn+2, Sn+2, Ni+2, and Se+2) and trivalent (Fe+3, As+3, and Al+3) metals on a mitochondrial preparation of K+-stimulated-p-nitrophenyl phosphatase (K+-PNPPase) from rat brain were studied. Except for salts of Ni+2, Se+2 and Li+1, which irrespective of concentration failed to produce 50% inhibition, all of the metals examined were found to be potent inhibitors of the enzyme with 150 values of 0.24 microM for Ag+1 among the monovalent, 0.70, 30, 37, 38, 47, 60, 62, 490 and 850 microM for Hg+2, Cu+2, Cd+2, Zn+2, Co+2, Fe+2, Pb+2, Mn+2 and Sn+2, respectively, among the divalents and 100, 550 and 870 microM for Fe+3, As+3, and AL+3 respectively, among the trivalents. Salts of silver and mercury were the most toxic for this enzyme. All metals showed concentration dependent inhibition except lithium. The order of their potency was Ag+1 greater than Hg+2 greater than Cu+2 greater than Cd+2 greater than Zn+2 greater than Co+2 greater than Fe+2 greater than Pb+2 greater than Fe+3 greater than Mn+2 greater than As+3 greater than Sn+2 greater than Al+3 greater than Ni+2 greater than Se+2 greater than Li+1.

摘要

研究了某些一价（Ag⁺¹和Li⁺¹）、二价（Hg⁺²、Cu⁺²、Zn⁺²、Co⁺²、Fe⁺²、Pb⁺²、Mn⁺²、Sn⁺²、Ni⁺²和Se⁺²）和三价（Fe⁺³、As⁺³和Al⁺³）金属对大鼠脑线粒体制备的钾离子刺激对硝基苯磷酸酶（K⁺-PNPPase）的影响。除了Ni⁺²、Se⁺²和Li⁺¹的盐，无论浓度如何都不能产生50%的抑制作用外，所有检测的金属都是该酶的有效抑制剂，一价金属中Ag⁺¹的I₅₀值为0.24微摩尔，二价金属中Hg⁺²、Cu⁺²、Cd⁺²、Zn⁺²、Co⁺²、Fe⁺²、Pb⁺²、Mn⁺²和Sn⁺²的I₅₀值分别为0.70、30、37、38、47、60、62、490和850微摩尔，三价金属中Fe⁺³、As⁺³和Al⁺³的I₅₀值分别为100、550和870微摩尔。银盐和汞盐对该酶毒性最大。除锂外，所有金属均表现出浓度依赖性抑制。它们的效力顺序为Ag⁺¹＞Hg⁺²＞Cu⁺²＞Cd⁺²＞Zn⁺²＞Co⁺²＞Fe⁺²＞Pb⁺²＞Fe⁺³＞Mn⁺²＞As⁺³＞Sn⁺²＞Al⁺³＞Ni⁺²＞Se⁺²＞Li⁺¹。