将小的质粒DNA片段从聚丙烯酰胺凝胶转移至硝酸纤维素纸上。
Transfer of small plasmid DNA fragments from polyacrylamide gels onto nitrocellulose paper.
作者信息
Frossard P, Sisco K L, Rucknagel D L
出版信息
Anal Biochem. 1983 Oct 15;134(2):265-8. doi: 10.1016/0003-2697(83)90295-6.
PMID:6316804
Abstract
The incorporation into a 7% polyacrylamide gel of a nucleic acid-specific photochemical reagent 4,5',8-trimethylpsoralen at a concentration of 1 mg/dl of acrylamide solution improves both the sensitivity and the efficiency of the transfer of plasmid DNA fragments from the gel onto nitrocellulose filters. This improvement allows detection by blot hybridization of DNA fragments as small as 57 bp long.
摘要
在7%的聚丙烯酰胺凝胶中加入浓度为1毫克/分升丙烯酰胺溶液的核酸特异性光化学试剂4,5',8-三甲基补骨脂素，可提高质粒DNA片段从凝胶转移到硝酸纤维素滤膜上的灵敏度和效率。这种改进使得通过印迹杂交能够检测到小至57碱基对长的DNA片段。
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