一种简单的分光光度法测定甲醛及其他醛类：应用于高碘酸盐氧化的二醇体系。
A simple spectrophotometric determination of formaldehyde and other aldehydes: application to periodate-oxidized glycol systems.
作者信息
Avigad G
出版信息
Anal Biochem. 1983 Oct 15;134(2):499-504. doi: 10.1016/0003-2697(83)90330-5.
PMID:6316809
Abstract
A simple, rapid, and sensitive spectrophotometric assay procedure for the determination of as low as 2 microM solutions of formaldehyde and acetaldehyde using an alkaline 4-amino-5-hydrazino-3-mercapto-1,2,4-triazole reagent is described. The method is particularly useful for determination of these aldehydes (0.5-50 nmol) when produced by the periodate oxidation of various glycols and can be applied to the assay of dilute solutions of sugars or polyols.
摘要
描述了一种简单、快速且灵敏的分光光度测定法，该方法使用碱性4-氨基-5-肼基-3-巯基-1,2,4-三唑试剂来测定低至2 microM的甲醛和乙醛溶液。当各种二醇经高碘酸盐氧化产生这些醛（0.5 - 50 nmol）时，该方法对于测定这些醛特别有用，并且可应用于糖或多元醇稀溶液的测定。
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