Mar E C, Cheng Y C, Huang E S
Antimicrob Agents Chemother. 1983 Oct;24(4):518-21. doi: 10.1128/AAC.24.4.518.
We studied the effect of a novel purine acyclic nucleoside, 9-(1,3-dihydroxy-2-propoxymethyl)guanine (DHPG), on human cytomegalovirus (HCMV) replication. The susceptibility of HCMV to this drug was monitored in cell culture by plaque reduction assay. HCMV replication of various strains was inhibited to the extent of 50% by 1 to 5 microM DHPG. DHPG was highly specific in its anti-HCMV activity, since at concentrations as high as 100 microM it did not exert any detectable inhibitory effect on uninfected cell macromolecular synthesis and cell growth. At concentrations of 2 to 4 microM, the drug inhibited the synthesis of six virus-specific polypeptides with molecular weights of 200,000 (VP200), 150,000 (VP150), 67,000 (VP67), 54,000 (VP54), 32,000 (VP32), and 27,000 (VP27) up to 96 h after infection. HCMV DNA synthesis was also considerably suppressed at concentrations of 2 to 4 microM DHPG. Upon removal of the inhibitor, however, viral DNA synthesis resumed and infectious virus reappeared, indicating that this inhibition was a virostatic reversible-type inhibition.
我们研究了一种新型嘌呤无环核苷9-(1,3-二羟基-2-丙氧甲基)鸟嘌呤（DHPG）对人巨细胞病毒（HCMV）复制的影响。通过蚀斑减少试验在细胞培养中监测HCMV对该药物的敏感性。1至5微摩尔的DHPG可将各种毒株的HCMV复制抑制50%。DHPG的抗HCMV活性具有高度特异性，因为在高达100微摩尔的浓度下，它对未感染细胞的大分子合成和细胞生长未产生任何可检测到的抑制作用。在2至4微摩尔的浓度下，该药物在感染后长达96小时内抑制了六种病毒特异性多肽的合成，这些多肽的分子量分别为200,000（VP200）、150,000（VP150）、67,000（VP67）、54,000（VP54）、32,000（VP32）和27,000（VP27）。在2至4微摩尔DHPG的浓度下，HCMV DNA合成也受到显著抑制。然而，去除抑制剂后，病毒DNA合成恢复，感染性病毒重新出现，表明这种抑制是一种病毒静止可逆型抑制。
