兔脊髓切片中促甲状腺激素释放激素（TRH）受体的生化与放射自显影研究

Biochemical and autoradiographic studies of TRH receptors in sections of rabbit spinal cord.

作者信息

Sharif N A, Pilotte N S, Burt D R

出版信息

Biochem Biophys Res Commun. 1983 Oct 31;116(2):669-74. doi: 10.1016/0006-291x(83)90577-6.

Abstract

Receptors for thyrotropin-releasing hormone (pGlu-His-Pro-NH2, TRH) on thaw-mounted sections of rabbit spinal cord have been identified biochemically and visualized by light microscopic autoradiography. Binding of [3H] [3-Me-His2]TRH to 20 microns sections exhibited high apparent affinity and a pharmacological specificity almost identical to that previously demonstrated for spinal TRH receptors in membranes. In autoradiograms, the highest density of TRH receptors appeared in the substantia gelatinosa of the dorsal gray and around the central canal, with intermediate levels in the ventral gray.

摘要

已通过生化方法鉴定了兔脊髓冰冻切片上促甲状腺激素释放激素（pGlu-His-Pro-NH2，TRH）的受体，并通过光学显微镜放射自显影进行了可视化。[3H][3-Me-His2]TRH与20微米切片的结合表现出高表观亲和力和药理学特异性，几乎与先前在膜中证明的脊髓TRH受体相同。在放射自显影片中，TRH受体的最高密度出现在背侧灰质的胶状质和中央管周围，腹侧灰质中的水平居中。