Manaker S, Winokur A, Rostene W H, Rainbow T C
J Neurosci. 1985 Jan;5(1):167-74. doi: 10.1523/JNEUROSCI.05-01-00167.1985.
We employed quantitative autoradiography to examine the distribution of thyrotropin-releasing hormone (TRH) receptors in the rat CNS. The binding of [3H]3-methyl-histidine-TRH [( 3H]MeTRH) to TRH receptors in frozen rat brain sections was saturable, of a high affinity (Kd = 5 nM), and specific for TRH analogs. Autoradiograms of [3H]MeTRH binding showed highest concentrations of TRH receptors in the rhinencephalon, including accessory olfactory bulb, nuclei of the amygdala, and the ventral dentate gyrus and subiculum of the hippocampus. Moderate TRH receptor concentrations were found within the thalamus and hypothalamus, in most regions of the rhombencephalon, such as the cranial nerve nuclei, and in the substantia gelatinosa of the spinal cord. Neocortex and basal ganglia contained low densities of TRH receptors. This distribution correlates well with the sensitivity of brain regions to the known effects of TRH, and suggests that TRH receptors may mediate the actions of TRH in the rat CNS.
我们采用定量放射自显影术来检测促甲状腺激素释放激素（TRH）受体在大鼠中枢神经系统中的分布。[3H]3-甲基组氨酸-TRH（[3H]MeTRH）与大鼠脑冰冻切片中TRH受体的结合具有饱和性、高亲和力（Kd = 5 nM），且对TRH类似物具有特异性。[3H]MeTRH结合的放射自显影片显示，在嗅脑，包括副嗅球、杏仁核核团以及海马体的腹侧齿状回和海马下托中，TRH受体浓度最高。在丘脑和下丘脑内、后脑的大多数区域（如脑神经核）以及脊髓的胶状质中发现了中等浓度的TRH受体。新皮质和基底神经节中TRH受体密度较低。这种分布与脑区对TRH已知作用的敏感性密切相关，并表明TRH受体可能介导TRH在大鼠中枢神经系统中的作用。
