人红细胞中皮质醇浓度的测定。

Determination of cortisol concentration in human erythrocytes.

作者信息

Hiramatsu R

出版信息

Clin Chim Acta. 1983 Oct 31;134(1-2):143-53. doi: 10.1016/0009-8981(83)90192-4.

Abstract

In order to investigate cytoplasmic free cortisol (F) concentrations, F levels in human red blood cells (RBC-F) were determined. Mean basal levels of plasma unbound free F and RBC-F in 10 normal healthy subjects were 16.6 and 14.8 ng/ml, respectively. RBC-F in the normal subjects showed well-defined diurnal rhythm, suppressibility by dexamethasone, and responsiveness to ACTH stimulation similar to plasma free F. There was an excellent linear relationship between RBC-F and corresponding plasma free F concentrations; RBC-F (ng/ml) = 1.25 X plasma free F (ng/ml) - 3.4, r = 0.94, n = 90. Mean basal RBC-F concentration in nine pregnant women was 13.9 ng/ml. In three patients with Cushing's syndrome and six patients with adrenal insufficiency, RBC-F showed parallel changes to plasma free F, which were apparently different from those in normal subjects, patients with simple obesity, and a patient with Cushing's disease in complete remission. These results indicated that RBC-F ran nearly parallel to plasma free F under various conditions.

摘要

为了研究细胞质游离皮质醇（F）浓度，测定了人红细胞中的F水平（RBC-F）。10名正常健康受试者血浆未结合游离F和RBC-F的平均基础水平分别为16.6和14.8 ng/ml。正常受试者的RBC-F表现出明确的昼夜节律、地塞米松可抑制性以及对促肾上腺皮质激素（ACTH）刺激的反应性，与血浆游离F相似。RBC-F与相应的血浆游离F浓度之间存在良好的线性关系；RBC-F（ng/ml）=1.25×血浆游离F（ng/ml）-3.4，r=0.94，n=90。9名孕妇的平均基础RBC-F浓度为13.9 ng/ml。在3例库欣综合征患者和6例肾上腺功能不全患者中，RBC-F与血浆游离F呈现平行变化，这明显不同于正常受试者、单纯肥胖患者以及完全缓解的库欣病患者。这些结果表明，在各种情况下，RBC-F与血浆游离F几乎平行变化。